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Anti-Phospho-E2F-1 (T433) antibody

[STJ90458] Download PDF Print Data Sheet
Anti-Phospho-E2F-1 (T433) antibody
5-7 days

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Product name Anti-Phospho-E2F-1 (T433) antibody
Short Description Rabbit polyclonal to Phospho-E2F-1 (T433).
Description E2F-1 is a protein encoded by the E2F1 gene which is approximately 46,9 kDa. E2F-1 is localised to the nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, glioma, E2F mediated regulation of DNA replication and activation of BH3-only proteins. It is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. E2F-1 is expressed in the skin, pancreas and nervous system. Mutations in the E2F1 gene may result in retinoblastoma and pharyngoconjunctival fever. STJ90458 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous E2F-1 protein which only binds about T433 when T433 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:5000
Specificity Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.
Protein Name Transcription factor E2F1
E2F-1
PBR3
Retinoblastoma-associated protein 1
RBAP-1
Retinoblastoma-binding protein 3
RBBP-3
pRB-binding protein E2F-1
Immunogen Synthesized peptide derived from human E2F-1 around the phosphorylation site of T433.
Immunogen Region 370-450 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1869
Gene Symbol E2F1
Molecular Weight 47 kDa
Database Links HGNC:3113
OMIM:189971
Alternative Names Anti-Phospho-E2F-1 (T433) antibody
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters .
Post-translational Modifications Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular Localization Nucleus.
Swiss-Prot Key Q01094_HUMAN
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