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Anti-Phospho-E2F-1 (T433) antibody

[STJ90458] Download PDF Print Data Sheet
Anti-Phospho-E2F-1 (T433) antibody

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Product name Anti-Phospho-E2F-1 (T433) antibody
Short Description Rabbit Polyclonal to Phospho-E2F-1 (T433).
Description E2F-1 is a protein encoded by the E2F1 gene which is approximately 46,9 kDa. E2F-1 is localised to the nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, glioma, E2F mediated regulation of DNA replication and activation of BH3-only proteins. It is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. E2F-1 is expressed in the skin, pancreas and nervous system. Mutations in the E2F1 gene may result in retinoblastoma and pharyngoconjunctival fever. STJ90458 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous E2F-1 protein which only binds about T433 when T433 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000 / ELISA 1:5000 /
Specificity Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.
Protein Name Transcription factor E2F1 - E2F1 - RBP3 - RBBP3 - E2F transcription factor 1
Immunogen Synthesized peptide derived from human E2F-1 around the phosphorylation site of T433.
Immunogen Region 370-450 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1869 (Human) / 13555 (Mouse) /
Gene Symbol E2F1
Molecular Weight 47 kDa
Database Links Human UniProt/Swiss-Prot:Q01094 Mouse UniPort/Swiss-Prot: Q61501 Rat UniProt/Swiss-Port: O09139 Human Entrez Gene: 1869 Mouse Entrez Gene: 13555 Rat Entrez Gene: Rn.72471
Alternative Names E2F1 / E2F transcription factor 1 / A306_00264 antibody / Anapl_11556 antibody / AS27_08275 antibody / AS28_13670 antibody / CB1_000408038 antibody / CG6376-PA antibody / CG6376-PB antibody / CG6376-PC antibody / CG6376-PD antibody / CG6376-PE antibody / CG6376-PF antibody / D623_10023721 antibody / dE2F antibody / dE2f1 antibody / def21 antibody / DmE2F-1 antibody / Dmel_CG6376 antibody / Dp antibody / drosE2F1 antibody / DRTF1 /E2F antibody / e2f antibody / E2F-1 antibody / E2f1-PA antibody / E2f1-PB antibody / E2f1-PC antibody / E2f1-PD antibody / E2f1-PE antibody / E2f1-PF antibody / E2F-1 transcription factor antibody / E2F transcription factor antibody / E2-promoter binding factor antibody / E(Sev-CycE)3A antibody / Evar(3)164 antibody / E(var)3-93E antibody / E(var)3-95E antibody / E(var)93E antibody / H920_07105 antibody / hypothetical protein antibody / I79_007483 antibody / M959_09551 antibody / MDA_GLEAN10022778 antibody / N300_14913 antibody / N301_10035 antibody / N302_02234 antibody / N303_01450 antibody / N305_05265 antibody / N307_12432 antibody / N309_03513 antibody / N311_05804 antibody / N320_08668 antibody / N325_07440 antibody / N326_06388 antibody / N330_10459 antibody / N331_09380 antibody / N335_05794 antibody / PAL_GLEAN10024239 antibody / PANDA_004504 antibody / PBR3 antibody / peroxisomal membrane protein 4 / 24kDa antibody / PRB-binding protein E2F-1 antibody / PXMP4 antibody / RBAP1 antibody / RBAP-1 antibody / RBBP3 antibody / RBBP-3 antibody / RBP3 antibody / retinoblastoma-associated protein 1 antibody / retinoblastoma-binding protein 3 antibody / Transcription factor E2F1 antibody / TREES_T100008613 antibody / UY3_04541 antibody / xE2F antibody / Y1Q_001163 antibody / Z169_05271 antibody
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters .
Post-translational Modifications Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. ; Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular Localization Nucleus.

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