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Anti-Phospho-EF-2 (T56) antibody

[STJ91030] Download PDF Print Data Sheet

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Product name Anti-Phospho-EF-2 (T56) antibody
Short Description Rabbit Polyclonal to Phospho-EF-2 (T56).
Description EF-2 is a protein encoded by the EEF2 gene which is approximately 95,3 kDa. EF-2 is localised to the cytoplasm and nucleus. It is involved in AMP-activated protein kinase signalling, viral mRNA translation, the innate immune system and the HIV life cycle. This protein falls under the GTP-binding translation elongation factor family. Its function is to catalyse the GTP dependent ribosomal translocation step during translation elongation. This involves the translocation of the nascent protein chain from the A-site to the P-site of the ribosome. EF-2 is expressed in the nervous system, liver, intestine, blood and lung. Mutations in the EEF2 gene may result in spinocerebellar ataxia and diphtheria. STJ91030 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous EF-2 protein which only binds about T56 when T56 is phosphorylated.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:10000 /
Specificity Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
Protein Name Elongation factor 2 - EEF2 - EEF-2 - EF2 - eukaryotic translation elongation factor 2
Immunogen Synthesized peptide derived from human EF-2 around the phosphorylation site of T56.
Immunogen Region 1-80 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1938 (Human) / 13629 (Mouse) / 29565 (Rat)
Gene Symbol EEF2
Molecular Weight 100 kDa
Database Links Human UniProt/Swiss-Prot:P13639 Mouse UniPort/Swiss-Prot: P58252 Rat UniProt/Swiss-Port: P05197 Human Entrez Gene: 1938 Mouse Entrez Gene: 13629 Rat Entrez Gene: Rn.55145
Alternative Names EEF2 / eukaryotic translation elongation factor 2 / A306_11346 antibody / AS27_08563 antibody / AS28_12383 antibody / CB1_000413032 antibody / Death-associated protein kinase 3 antibody / EEF-2 antibody / EF2 antibody / EF-2 antibody / elongation factor 2 antibody / elongation factor-2 antibody / ETS-related factor2 antibody / eukaryotic elongation factor 2 antibody / GW7_06774 antibody / H920_09785 antibody / hypothetical protein antibody / I79_013848 antibody / M91_12003 antibody / M959_13666 antibody / MDA_GLEAN10011115 antibody / N300_08720 antibody / N301_05877 antibody / N302_03627 antibody / N303_12063 antibody / N305_10273 antibody / N306_10099 antibody / N307_01292 antibody / N308_14404 antibody / N309_13852 antibody / N310_02291 antibody / N311_00121 antibody / N321_04101 antibody / N324_07839 antibody / N325_09591 antibody / N326_01708 antibody / N327_05584 antibody / N330_08364 antibody / N331_00370 antibody / N332_05521 antibody / N334_10397 antibody / N335_07866 antibody / N336_10183 antibody / N339_05508 antibody / PAL_GLEAN10006050 antibody / PANDA_018484 antibody / polypeptidyl-tRNA translocase antibody / SCA26 antibody / UY3_18886 antibody / Y1Q_025771 antibody / Y956_14260 antibody / Z169_09715 antibody / zef2 antibody
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Post-translational Modifications Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. ; Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity). ; ISGylated. ; Proteolytically processed at two sites following phosphorylation by CSK. ; SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Cellular Localization Cytoplasm Nucleus Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.

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