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Anti-Phospho-EGFR (S1071) antibody

[STJ90568] Download PDF Print Data Sheet
Anti-Phospho-EGFR (S1071) antibody

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Product name Anti-Phospho-EGFR (S1071) antibody
Short Description Rabbit Polyclonal to Phospho-EGFR (S1071).
Description EGFR is a protein encoded by the EGFR gene which is approximately 134,3 kDa. EGFR is localised to the cell membrane. and endoplasmic reticulum membrane. It is involved in RET signalling, apoptotic pathways in synovial fibroblasts, glioma and the GPCR pathway. It is a transmembrane glycoprotein that is a member of the protein kinase superfamily. It binds to epidermal growth factor and this induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. EGFR is expressed ubiquitously with isoform 2 also being expressed in ovarian cancers. Mutations in the EGFR gene may result in lung cancer and Cowden syndrome. STJ90568 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous EGFR protein which only binds about S1071 when S1071 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000 / ELISA 1:5000 /
Specificity Phospho-EGFR (S1071) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at S1071.
Protein Name Epidermal growth factor receptor - EGFR - ERBB1 - ERBB - epidermal growth factor receptor
Immunogen Synthesized peptide derived from human EGFR around the phosphorylation site of S1071.
Immunogen Region 1010-1090 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1956 (Human) / 13649 (Mouse) /
Gene Symbol EGFR
Molecular Weight 150-160 kDa
Database Links Human UniProt/Swiss-Prot:P00533 Mouse UniPort/Swiss-Prot: Q01279 Rat UniProt/Swiss-Port: Q9QX70 Human Entrez Gene: 1956 Mouse Entrez Gene: 13649 Rat Entrez Gene: Rn.37227
Alternative Names EGFR / epidermal growth factor receptor / A306_05579 antibody / AGAP008819-PA antibody / AS27_07788 antibody / AS28_09817 antibody / avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody / avian erythroblastic leukemia viral (v-erbB) oncogene homolog antibody / cell growth inhibiting protein 40 antibody / cell proliferation-inducing protein 61 antibody / CER antibody / C-erb antibody / c-erbB antibody / CG10079-PA antibody / CG10079-PB antibody / CGI_10005663 antibody / D-Egf antibody / degfr antibody / d-egf-r antibody / D-EGFR antibody / dEGFR1 antibody / der antibody / DER1 antibody / DER /EGFR antibody / DER-Ellipse antibody / DER flb antibody / DER /top antibody / DER /torpedo antibody / Dmel_CG10079 antibody / DmHD-33 antibody / drosophila epidermal growth factor receptor homologue antibody / EC2-4 antibody / EFG-R antibody / Egf antibody / EGF-R antibody / Egf receptor antibody / EGF-receptor antibody / Egfr-PA antibody / Egfr-PB antibody / EGFR-related peptide antibody / EK2-6 antibody / El antibody / ellipse antibody / ellipse torpedo antibody / Elp antibody / Elp-1 antibody / Elp-B1 antibody / Elp-B1RB1 antibody / ENSANGG00000016316 antibody / epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog / avian) antibody / epidermal growth factor receptor / formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1) antibody / Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1) antibody / erbb antibody / erbb1 antibody / ErbB-1 antibody / erb-b2 receptor tyrosine kinase 1 antibody / Errb1 antibody / Errp antibody / faint little ball antibody / Flb antibody / GW7_07013 antibody / H920_05628 antibody / HD-33 antibody / HER1 antibody / hypothetical protein antibody / I79_008941 antibody / M959_01595 antibody / MDA_GLEAN10022029 antibody / mENA antibody / mor1 antibody / morphological defects 1 antibody / N300_15066 antibody / N301_16722 antibody / N302_15981 antibody / N303_08901 antibody / N305_04968 antibody / N307_01718 antibody / N308_04589 antibody / N309_03195 antibody / N310_13526 antibody / N311_06960 antibody / N312_12049 antibody / N320_12361 antibody / N321_07878 antibody / N322_00598 antibody / N324_11117 antibody / N325_00562 antibody / N326_05961 antibody / N327_06688 antibody / N328_04976 antibody / N329_01993 antibody / N330_03652 antibody / N331_05707 antibody / N332_02117 antibody / N335_12363 antibody / N336_09203 antibody / N339_12809 antibody / N340_04849 antibody / NISBD2 antibody / PAL_GLEAN10021992 antibody / PIG61 antibody / proto-oncogene c-ErbB-1 antibody / receptor tyrosine kinase ErbB1 antibody / receptor tyrosine-protein kinase erbB-1 antibody / top antibody / top /DER antibody / top /flb antibody / Tor antibody / torpedo antibody / Torpedo /DER antibody / torpedo /Egfr antibody / TREES_T100003987 antibody / UY3_06630 antibody / wa2 antibody / wa-2 antibody / Wa5 antibody / waved 2 antibody / x-agfrA antibody / XEgfr antibody / x-egfrA protein (partial) antibody / Y1Q_028180 antibody / Y956_15238 antibody
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity). ; Isoform 2 may act as an antagonist of EGF action.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Post-translational Modifications Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. ; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity). ; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Cellular Localization Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).; Isoform 2: Secreted.
Tissue Specificity Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

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