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Anti-Phospho-ERK 1/2 (T202/Y204) antibody

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Product name Anti-Phospho-ERK 1/2 (T202/Y204) antibody
Description Rabbit Polyclonal to Phospho-ERK 1/2 (T202/Y204).
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC, WB
Immunogen Synthesized peptide derived from human ERK 1/2 around the phosphorylation site of T202 and Y204.
Gene ID 5595/5594 (Human) / 26417/26413 (Mouse) / 50689/116590 (Rat)
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:20000 /
Specificity Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202 or Y204.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note For Research Use Only (RUO).
Protein Name Mitogen-activated protein kinase 3 - MAPK3 - ERK1 - p44mapk - p44erk1 - PRKM3 - mitogen-activated protein kinase 3
Clonality Polyclonal
Conjugation Unconjugated
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links Human UniProt/Swiss-Prot:P27361 Human Entrez Gene: 5595
Alternative Names MAPK3 / mitogen-activated protein kinase 3 / CB1_000739027 antibody / D623_10014441 antibody / Erk-1 antibody / ERK1 antibody / Ert2 antibody / Esrk1 antibody / extracellular signal-regulated kinase 1 antibody / extracellular signal-regulated kinase-1 antibody / extracellular signal-related kinase 1 antibody / fi06b09 antibody / H920_02263 antibody / HS44KDAP antibody / HUMKER1A antibody / hypothetical protein antibody / I79_018350 antibody / insulin-stimulated MAP2 kinase antibody / M91_15159 antibody / MAPK1 antibody / MAPK 1 antibody / MAPK 3 antibody / MAP kinase 1 antibody / MAP kinase 3 antibody / MAP kinase-activated protein kinase 3 antibody / MAP kinase isoform p44 antibody / MDA_GLEAN10001861 antibody / microtubule-associated protein 2 kinase antibody / mitogen-activated 3 antibody / mitogen-activated protein kinase 1 antibody / mitogen activated protein kinase 3 antibody / Mnk1 antibody / Mtap2k antibody / p44 antibody / p44-ERK1 antibody / P44ERK1 antibody / p44-MAPK antibody / P44MAPK antibody / p44 MAP kinase antibody / PAL_GLEAN10011855 antibody / PANDA_017159 antibody / pp42 /MAP kinase antibody / Prkm3 antibody / TREES_T100000436 antibody / zERK1 antibody
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational Modifications Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
Cellular Localization Cytoplasm. Nucleus. Membrane, caveola Note=Autophosphorylation at Thr-207 promotes nuclear localization.

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