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Anti-Phospho-ERK 1/2 (Y222/205) antibody

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Product name Anti-Phospho-ERK 1/2 (Y222/205) antibody
Description Rabbit Polyclonal to Phospho-ERK 1/2 (Y222/205).
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Immunogen Synthesized peptide derived from human ERK 1/2 around the phosphorylation site of Y222/205.
Gene ID 5594/5595 (Human) / 26413/26417 (Mouse) / 116590/50689 (Rat)
Dilution range WB 1:500-1:2000 / ELISA 1:10000 /
Specificity Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y222/205.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note For Research Use Only (RUO).
Protein Name Mitogen-activated protein kinase 1 - MAPK1 - ERK - ERK2 - p41mapk - MAPK2 - PRKM2 - PRKM1 - mitogen-activated protein kinase 1
Clonality Polyclonal
Conjugation Unconjugated
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links Human UniProt/Swiss-Prot:P28482 Human Entrez Gene: 5594
Alternative Names MAPK1 / mitogen-activated protein kinase 1 / A306_02170 antibody / Anapl_06912 antibody / AS28_08463 antibody / C78273 antibody / CB1_000302020 antibody / D623_10021792 antibody / EH28_06814 antibody / erk antibody / ERK2 antibody / ERK-2 antibody / ert1 antibody / extracellular regulated protein 2 antibody / extracellular signal-regulated kinase 2 antibody / extracellular signal-regulated kinase-2 antibody / extracellular-signal-regulated kinase 2 antibody / H920_03835 antibody / hypothetical protein antibody / I79_009500 antibody / M91_15504 antibody / M959_10212 antibody / mapk antibody / MAPK 1 antibody / MAPK2 antibody / MAPK 2 antibody / MAP kinase antibody / MAP kinase 1 antibody / MAP kinase 2 antibody / MAP kinase isoform p42 antibody / MDA_GLEAN10020149 antibody / mitogen activated protein kinase 1 antibody / Mitogen-activated protein kinase 1 isoform 3-like protein antibody / mitogen-activated protein kinase 2 antibody / mpk1 antibody / N300_08178 antibody / N301_08299 antibody / N302_01461 antibody / N303_09680 antibody / N305_01931 antibody / N306_08529 antibody / N307_14107 antibody / N308_04257 antibody / N309_03772 antibody / N311_04993 antibody / N320_04113 antibody / N321_07948 antibody / N322_06382 antibody / N324_02682 antibody / N326_08132 antibody / N328_07440 antibody / N331_11802 antibody / N334_05011 antibody / N335_13422 antibody / N336_05235 antibody / p38 antibody / p40 antibody / p41 antibody / p41mapk antibody / p42mapk antibody / p42-MAPK antibody / PAL_GLEAN10010848 antibody / PANDA_015979 antibody / Prkm1 antibody / prkm2 antibody / protein tyrosine kinase ERK2 antibody / UY3_02436 antibody / xp42 antibody / Y1Q_010249 antibody / Z169_05497 antibody / zERK2 antibody
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity). ; Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational Modifications Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. ; ISGylated.
Cellular Localization Cytoplasm, cytoskeleton, spindle Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Membrane, caveola Note=Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.

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