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Anti-Phospho-FoxO3A (S253) antibody

[STJ90275] Download PDF Print Data Sheet

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Product name Anti-Phospho-FoxO3A (S253) antibody
Short Description Rabbit Polyclonal to Phospho-FoxO3A (S253).
Description FoxO3A is a protein encoded by the FOXO3 gene which is approximately 71,2 kDa. FoxO3A is localised to the cytoplasm and nucleus. It is involved in RET signalling, GAB1 signalosome, glioma and common cytokine receptor gamma-chain family signalling pathways. It is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It also participates in post-transcriptional regulation of MYC. FoxO3A is expressed ubiquitously. Mutations in the FOXO3 gene may result in secondary acute leukemia. STJ90275 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous FoxO3A protein which only binds about S253 when S253 is phosphorylated.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:20000 /
Specificity Phospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.
Protein Name Forkhead box protein O3 - FOXO3 - AF6q21 - FOXO2 - FKHRL1 - FOXO3A - forkhead box O3
Immunogen Synthesized peptide derived from human FoxO3A around the phosphorylation site of S253.
Immunogen Region 190-270 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2309 (Human) / 56484 (Mouse) /
Gene Symbol FOXO3
Molecular Weight 71 kDa
Database Links Human UniProt/Swiss-Prot:O43524 Mouse UniPort/Swiss-Prot: Q9WVH4 Rat UniProt/Swiss-Port: D3ZBQ1 Human Entrez Gene: 2309 Mouse Entrez Gene: 56484 Rat Entrez Gene: Rn.165338
Alternative Names FOXO3 / forkhead box O3 / A306_08843 antibody / AF6q21 antibody / C76856 antibody / CB1_000993032 antibody / D623_10034673 antibody / Fkhr2 antibody / FKHRL1 antibody / FKHRL1P2 antibody / forkhead box O3A antibody / forkhead box O3A isoform 1-like protein antibody / forkhead box O3A transcription factor antibody / forkhead box O3b antibody / forkhead box O5 antibody / forkhead box O protein antibody / forkhead box protein O3 antibody / forkhead box protein O3 transcript variant X1 antibody / forkhead box protein O3 transcript variant X2 antibody / forkhead / Drosophila / homolog of / in rhabdomyosarcoma-like 1 antibody / forkhead homolog (rhabdomyosarcoma) like 1 antibody / forkhead in rhabdomyosarcoma-like 1 antibody / forkhead protein FKHR2 antibody / forkhead protein FoxO5 antibody / foxO antibody / FOXO2 antibody / FOXO-3 antibody / Foxo3a antibody / FOXO3B antibody / FoxO5 antibody / GW7_16339 antibody / H920_09948 antibody / hypothetical protein antibody / M91_06041 antibody / PAL_GLEAN10018577 antibody / PANDA_005606 antibody / TREES_T100016244 antibody / UY3_04791 antibody / xFoxO3 antibody / Y1Q_019513 antibody
Function Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Post-translational Modifications In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation . Phosphorylated by CHUK/IKKA and IKBKB/IKKB. TNF-induced inactivation of FOXO3 requires its phosphorylation at Ser-644 by IKBKB/IKKB which promotes FOXO3 retention in the cytoplasm, polyubiquitination and ubiquitin-mediated proteasomal degradation . May be dephosphorylated by calcineurin A on Ser-299 which abolishes FOXO3 transcriptional activity (By similarity). ; Deacetylation by SIRT1 or SIRT2 stimulates interaction of FOXO3 with SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation . Deacetylation by SIRT2 stimulates FOXO3-mediated transcriptional activity in response to oxidative stress (By similarity). ; Heavily methylated by SET9 which decreases stability, while moderately increasing transcriptional activity. The main methylation site is Lys-271. Methylation doesn't affect subcellular location. ; Polyubiquitinated. Ubiquitinated by a SCF complex containing SKP2, leading to proteasomal degradation.
Cellular Localization Cytoplasm, cytosol Nucleus Note=Retention in the cytoplasm contributes to its inactivation. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
Tissue Specificity Ubiquitous.

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