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Anti-Phospho-Histone H3 (S10) antibody

[STJ90289] Download PDF Print Data Sheet

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Product name Anti-Phospho-Histone H3 (S10) antibody
Short Description Rabbit Polyclonal to Phospho-Histone H3 (S10).
Description Histone H3 is a protein encoded by the HIST3H3 gene which is approximately 15,5 kDa. Histone H3 is localised to the nucleus and chromosome. It is involved in the DNA double-strand break repair and mitotic cell cycle. This protein falls under the histone family protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 is expressed in the testicular cells. STJ90289 was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody specifically binds to endogenous Histone H3 protein which only binds about S10 when S10 is phosphorylated.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:10000 /
Specificity Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.
Protein Name Histone H3.1 - HIST1H3A - H3/A - H3FA - histone cluster 1 H3 family member a
Immunogen Synthesized peptide derived from human Histone H3 around the phosphorylation site of S10.
Immunogen Region 1-80 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 (Human) / 319152/15077/15078 (Mouse) / 291159/100361558 (Rat)
Gene Symbol HIST1H3A
Molecular Weight 17 kDa
Database Links Human UniProt/Swiss-Prot:P68431 Human Entrez Gene: 8350
Alternative Names HIST1H3A / histone cluster 1 / H3a / H3 /A antibody / H3F3A antibody / H3FA antibody / H3 histone / family 3A antibody / H3 histone family / member A antibody / histone 1 / H3a antibody / histone H3.2-like antibody / histone H3 /a antibody / null antibody

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