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Anti-Phospho-Histone H3 (S10) antibody

[STJ90289] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Phospho-Histone H3 (S10) antibody
Short Description Rabbit polyclonal to Phospho-Histone H3 (S10).
Description Histone H3 is a protein encoded by the HIST3H3 gene which is approximately 15,5 kDa. Histone H3 is localised to the nucleus and chromosome. It is involved in the DNA double-strand break repair and mitotic cell cycle. This protein falls under the histone family protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 is expressed in the testicular cells. STJ90289 was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody specifically binds to endogenous Histone H3 protein which only binds about S10 when S10 is phosphorylated.
Applications ELISA, IF, IHC-p, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.
Protein Name Histone H3.1
Histone H3/a
Histone H3/b
Histone H3/c
Histone H3/d
Histone H3/f
Histone H3/h
Histone H3/i
Histone H3/j
Histone H3/k
Histone H3/l
Immunogen Synthesized peptide derived from human Histone H3 around the phosphorylation site of S10.
Immunogen Region 1-80 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 8350
Gene Symbol HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J
Molecular Weight 17 kDa
Database Links HGNC:4766
OMIM:137800
Alternative Names Anti-Phospho-Histone H3 (S10) antibody
Swiss-Prot Key P68431_HUMAN
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