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Anti-Phospho-IκB-α (Y305) antibody

[STJ90912] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Phospho-IκB-α (Y305) antibody
Short Description Rabbit polyclonal to Phospho-IκB-α (Y305).
Description IκB-α is a protein encoded by the NFKBIA gene which is approximately 35,6 kDa. IκB-α is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, the 4-1BB pathway and toll-like receptor signalling pathways. This protein falls under the NF-kappa-B inhibitor family. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals, this process is involved in inflammatory responses. IκB-α is induced in adherent monocytes. Mutations in the NFKBIA gene may result in ectodermal dysplasia with T-cell immunodeficiency. STJ90912 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IκB-α protein which only binds about Y305 when Y305 is phosphorylated.
Applications ELISA, IHC-p, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Specificity Phospho-IκB-α (Y305) Polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at Y305.
Protein Name NF-kappa-B inhibitor alpha
I-kappa-B-alpha
IkB-alpha
IkappaBalpha
Major histocompatibility complex enhancer-binding protein MAD3
Immunogen Synthesized peptide derived from human IκB-α around the phosphorylation site of Y305.
Immunogen Region 240-320 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Monkey, Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4792
Gene Symbol NFKBIA
Molecular Weight 40 kDa
Database Links HGNC:7797
OMIM:164008
Alternative Names Anti-Phospho-IB-a (Y305) antibody
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Post-translational Modifications Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
Cellular Localization Cytoplasm. Nucleus.. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Swiss-Prot Key P25963_HUMAN
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