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Anti-Phospho-IκB-α (Y305) antibody

[STJ90912] Download PDF Print Data Sheet

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Product name Anti-Phospho-IκB-α (Y305) antibody
Short Description Rabbit Polyclonal to Phospho-IκB-α (Y305).
Description IκB-α is a protein encoded by the NFKBIA gene which is approximately 35,6 kDa. IκB-α is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, the 4-1BB pathway and toll-like receptor signalling pathways. This protein falls under the NF-kappa-B inhibitor family. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals, this process is involved in inflammatory responses. IκB-α is induced in adherent monocytes. Mutations in the NFKBIA gene may result in ectodermal dysplasia with T-cell immunodeficiency. STJ90912 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IκB-α protein which only binds about Y305 when Y305 is phosphorylated.
Applications IHC-p, ELISA, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:5000 /
Specificity Phospho-IκB-α (Y305) Polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at Y305.
Protein Name NF-kappa-B inhibitor alpha - NFKBIA - IKBA - MAD-3 - IkappaBalpha - NFKBI - NFKB inhibitor alpha
Immunogen Synthesized peptide derived from human IκB-α around the phosphorylation site of Y305.
Immunogen Region 240-320 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Monkey, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4792 (Human) / 18035 (Mouse) / 25493 (Rat)
Gene Symbol NFKBIA
Molecular Weight 40 kDa
Database Links Human UniProt/Swiss-Prot:P25963 Mouse UniPort/Swiss-Prot: Q9Z1E3 Rat UniProt/Swiss-Port: Q63746 Human Entrez Gene: 4792 Mouse Entrez Gene: 18035 Rat Entrez Gene: Rn.12550
Alternative Names NFKBIA / NFKB inhibitor alpha / A306_08088 antibody / AS27_00490 antibody / AS28_13511 antibody / CB1_000886003 antibody / D623_10021676 antibody / ECI-6 antibody / ECI-6 /IKBA antibody / H920_09334 antibody / I79_018146 antibody / ikappaBalpha antibody / I kappa B-alpha antibody / I-kappaBalpha antibody / I-kappa-B-alpha antibody / IkappaB alpha antibody / I(Kappa)B(alpha) antibody / IKBA antibody / ikB-alpha antibody / Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha antibody / Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells / alpha antibody / M959_01075 antibody / MAD-3 antibody / major histocompatibility complex enhancer-binding protein MAD3 antibody / MDA_GLEAN10008306 antibody / N300_05692 antibody / N301_02121 antibody / N302_01755 antibody / N303_01788 antibody / N305_01107 antibody / N306_10576 antibody / N307_10216 antibody / N308_05024 antibody / N309_05009 antibody / N310_01585 antibody / N311_03728 antibody / N312_13097 antibody / N320_04147 antibody / N321_02260 antibody / N322_05237 antibody / N324_00372 antibody / N325_10336 antibody / N327_12588 antibody / N328_06661 antibody / N329_08565 antibody / N330_02009 antibody / N331_00453 antibody / N332_01757 antibody / N333_03000 antibody / N334_04614 antibody / N335_02116 antibody / N336_00171 antibody / N339_09122 antibody / N340_10449 antibody / N341_10196 antibody / NF-kappa-B inhibitor alpha antibody / NF-kappaB inhibitor alpha antibody / Nfkbi antibody / NF-kB inhibitor alpha antibody / nuclear factor of kappa light chain gene enhancer in B-cells antibody / nuclear factor of kappa light chain gene enhancer in B-cells inhibitor / alpha antibody / nuclear factor of kappa light polypeptide gene enhancer in B-cel antibody / nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor / alpha antibody / nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha antibody / nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor / alpha antibody / nuclear factor of kappa light polypeptide protein enhancer in B-cells inhibitor / alpha antibody / nuclear factor of kappa light polyp gene enhancer in B-cell 1 antibody / null antibody / PAL_GLEAN10008489 antibody / Rel-associated pp40 antibody / REL-associated protein pp40 antibody / RL /IF-1 antibody / TREES_T100010775 antibody / Y1Q_018434 antibody / Y956_04740 antibody / Z169_15383 antibody
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Post-translational Modifications Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. ; Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. ; Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. ; Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
Cellular Localization Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

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