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Anti-Phospho-IRF-3 (S385) antibody

[STJ91040] Download PDF Print Data Sheet

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Product name Anti-Phospho-IRF-3 (S385) antibody
Short Description Rabbit Polyclonal to Phospho-IRF-3 (S385).
Description IRF-3 is a protein encoded by the IRF3 gene which is approximately 47,2 kDa. IRF-3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling and toll-like receptor signalling pathway. This protein falls under the interferon regulatory transcription factor family. It is found in an inactive form in the cytoplasm that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex is then translocated to the nucleus where it activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 is expressed constitutively in a variety of tissues. Mutations in the IRF3 gene may be involved in herpes simplex encephalitis. STJ91040 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IRF-3 protein which only binds about S385 when S385 is phosphorylated.
Applications IHC-p, ELISA, IF, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:10000 /
Specificity Phospho-IRF-3 (S385) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S385.
Protein Name Interferon regulatory factor 3 - IRF3 - interferon regulatory factor 3
Immunogen Synthesized peptide derived from human IRF-3 around the phosphorylation site of S385.
Immunogen Region 320-400 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 3661 (Human) /
Gene Symbol IRF3
Molecular Weight 60 kDa
Database Links Human UniProt/Swiss-Prot:Q14653 Mouse UniPort/Swiss-Prot: P70671 Rat UniProt/Swiss-Port: Q5XIB0 Human Entrez Gene: 3661 Mouse Entrez Gene: 54131 Rat Entrez Gene: Rn.1499
Alternative Names IRF3 / interferon regulatory factor 3 / CB1_000132021 antibody / D623_10017659 antibody / GW7_15298 antibody / H920_12363 antibody / hypothetical protein antibody / I79_023218 antibody / IIAE7 antibody / IRF-3 antibody / MDA_GLEAN10005847 antibody / PAL_GLEAN10004537 antibody / PANDA_006263 antibody / TREES_T100015231 antibody / Y1Q_018247 antibody
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Post-translational Modifications Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. ; (Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. ; Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. ; ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
Cellular Localization Cytoplasm Nucleus Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Tissue Specificity Expressed constitutively in a variety of tissues.


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