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Anti-Phospho-JNK1/2/3 (T183/Y185) antibody

[STJ90317] Download PDF Print Data Sheet

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Product name Anti-Phospho-JNK1/2/3 (T183/Y185) antibody
Short Description Rabbit Polyclonal to Phospho-JNK1/2/3 (T183/Y185).
Description JNK1/2/3 is a protein encoded by the MAPK8 gene which is approximately 48,2 kDa. JNK1/2/3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, TNFR1 pathway, toll-like receptor signalling pathways and the 4-1BB pathway. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, therefore mediating immediate-early gene expression in response to cell stimuli. JNK1/2/3 is expressed in the nervous system, liver, blood, heart and lung. Mutations in the MAPK8 gene may result in fatty liver disease and renal fibrosis. STJ90317 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous JNK1/2/3 protein which only binds about T183/Y185 when T183/Y185 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000 / ELISA 1:10000 /
Specificity Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185.
Protein Name Mitogen-activated protein kinase 8/9/10 - MAPK8 - JNK - JNK1 - SAPK1 - PRKM8 - mitogen-activated protein kinase 8
Immunogen Synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of T183/Y185.
Immunogen Region 120-200 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Chicken, Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5599/5601/5602 (Human) / 26419/26420 (Mouse) / 116554/50658/25272 (Rat)
Gene Symbol MAPK8
Molecular Weight 48 kDa
Database Links Human UniProt/Swiss-Prot:P45983 Human Entrez Gene: 5599
Alternative Names MAPK8 / mitogen-activated protein kinase 8 / Anapl_09169 antibody / AS27_02584 antibody / AS28_04483 antibody / c-jun NH2-terminal kinase antibody / c-Jun N-terminal kinase 1 antibody / D623_10008765 antibody / GW7_11354 antibody / H920_04623 antibody / janus kinase 1 antibody / jnk antibody / JNK1 antibody / JNK1A2 antibody / JNK1 beta1 protein kinase antibody / JNK21B1 /2 antibody / JNK-46 antibody / JUN N-terminal kinase antibody / M959_03218 antibody / MAPK 8 antibody / MAP kinase 8 antibody / MDA_GLEAN10013122 antibody / mitogen activated protein kinase 8 antibody / mitogen-activated protein kinase 8 isoform alpha2 antibody / mitogen-activated protein kinase 8 isoform beta2 antibody / mitogen-activated protein kinase 8 isoform JNK1 alpha1 antibody / mitogen-activated protein kinase 8 isoform JNK1 alpha2 antibody / mitogen-activated protein kinase 8 isoform JNK1 beta1 antibody / mitogen-activated protein kinase 8 isoform JNK1 beta2 antibody / N300_07524 antibody / N301_10666 antibody / N302_13468 antibody / N303_09210 antibody / N305_11440 antibody / N306_13178 antibody / N307_06121 antibody / N308_09059 antibody / N310_06203 antibody / N311_12939 antibody / N312_10444 antibody / N320_02500 antibody / N321_05396 antibody / N322_06980 antibody / N324_08591 antibody / N325_00856 antibody / N327_04793 antibody / N328_08012 antibody / N330_03964 antibody / N333_06039 antibody / N335_01652 antibody / N336_12658 antibody / N339_08237 antibody / N340_12458 antibody / N341_11211 antibody / p54 gamma antibody / PAL_GLEAN10004413 antibody / PANDA_014394 antibody / Prkm8 antibody / protein kinase mitogen-activated 8 antibody / sapk1 antibody / SAPK1c antibody / SAPK gamma antibody / stress-activated protein kinase 1 antibody / stress-activated protein kinase 1c antibody / stress-activated protein kinase JNK1 antibody / TREES_T100013923 antibody / UY3_15889 antibody / Y1Q_010487 antibody / Y956_01539 antibody / Z169_12792 antibody
Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock . Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity . ; JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Post-translational Modifications Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
Cellular Localization Cytoplasm Nucleus
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

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