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Anti-phospho-MLKL (S358) antibody

[STJ97776] Download PDF Print Data Sheet

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Product name Anti-phospho-MLKL (S358) antibody
Short Description Mouse Monoclonal to phospho-MLKL (S358).
Description MLKL is a protein encoded by the MLKL gene which is approximately 54,4 kDa. MLKL is localised to the cell membrane and cytoplasm. It is involved in the dimerization of procaspase-8, the TNF signalling pathway, apoptosis and autophagy. This protein falls under the protein kinase superfamily. It plays a critical role in tumour necrosis factor-induced necroptosis which is a programmed cell death process. It does this via interaction with the receptor-interacting protein 3 which is a key signalling molecule in necroptosis pathway. MLKL is expressed in the heart, intestine and lung. STJ97776 was developed from clone 6F8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen MLKL. This primary antibody specifically binds to endogenous MLKL protein which only binds about S358 when S358 is phosphorylated.
Applications IHC-p
Dilution range IHC 1:100-200
Specificity phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8) detects endogenous levels of MLKL
Protein Name MLKL - MLKL - FLJ34389 - mixed lineage kinase domain like pseudokinase
Immunogen synthetic peptide derived from phospho-MLKL (S358)
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 6F8
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 197259 (Human)
Gene Symbol MLKL
Database Links Human UniProt/Swiss-Prot:Q8NB16 Mouse UniPort/Swiss-Prot: Q9D2Y4 Rat UniProt/Swiss-Port: D3ZKP6 Human Entrez Gene: 197259 Mouse Entrez Gene: 74568
Alternative Names MLKL / mixed lineage kinase domain-like / Anapl_07481 antibody / AS27_01878 antibody / AS28_08004 antibody / CB1_000407005 antibody / D623_10035459 antibody / EGK_12996 antibody / hMLKL antibody / M91_18478 antibody / M959_02031 antibody / MDA_GLEAN10016756 antibody / mixed lineage kinase domain-like protein antibody / mixed lineage kinase domain-like protein-like antibody / N300_08887 antibody / N301_02343 antibody / N302_07016 antibody / N303_05081 antibody / N305_06415 antibody / N306_03199 antibody / N307_12377 antibody / N308_13539 antibody / N310_08551 antibody / N311_07278 antibody / N312_08288 antibody / N320_10091 antibody / N321_06594 antibody / N322_08851 antibody / N324_06059 antibody / N325_04400 antibody / N327_06823 antibody / N328_06051 antibody / N329_09144 antibody / N330_09066 antibody / N331_06084 antibody / N332_07121 antibody / N334_03601 antibody / N335_07276 antibody / N336_06568 antibody / N339_07350 antibody / N340_06753 antibody / N341_03911 antibody / rCG51567-like antibody / UY3_17961 antibody / Y956_09290 antibody / Z169_06063 antibody
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Post-translational Modifications Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Cellular Localization Cytoplasm Cell membrane Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Sequence and Domain Family The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity). ; The coiled coil region 2 is responsible for homotrimerization.


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