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Anti-Phospho-MSK1 (S360) antibody

[STJ91293] Download PDF Print Data Sheet
Anti-Phospho-MSK1 (S360) antibody

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Product name Anti-Phospho-MSK1 (S360) antibody
Short Description Rabbit Polyclonal to Phospho-MSK1 (S360).
Description MSK1 is a protein encoded by the RPS6KA5 gene which is approximately 89,8 kDa. MSK1 is localised to the nucleus and cytoplasm. It is involved in activated TLR4 signalling, ERK signalling and RET signalling. It is a serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81. It also contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. MSK1 is widely expressed with high levels in heart, brain and placenta. STJ91293 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MSK1 protein which only binds about S360 when S360 is phosphorylated.
Applications IHC-p, ELISA, IF
Dilution range IHC 1:100-1:300 / IF 1:200-1:1000 / ELISA 1:10000 /
Specificity Phospho-MSK1 (S360) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S360.
Protein Name Ribosomal protein S6 kinase alpha-5 - RPS6KA5 - MSK1 - RLPK - ribosomal protein S6 kinase A5
Immunogen Synthesized peptide derived from human MSK1 around the phosphorylation site of S360.
Immunogen Region 300-380 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 9252 (Human) / 73086 (Mouse) /
Gene Symbol RPS6KA5
Molecular Weight 90 kDa
Database Links Human UniProt/Swiss-Prot:O75582 Mouse UniPort/Swiss-Prot: Q8C050 Rat UniProt/Swiss-Port: D3ZY17 Human Entrez Gene: 9252 Mouse Entrez Gene: 73086 Rat Entrez Gene: Rn.8940
Alternative Names RPS6KA5 / ribosomal protein S6 kinase A5 / 90 kDa ribosomal protein S6 kinase 5 antibody / A306_03343 antibody / AS27_04543 antibody / AS28_13631 antibody / CB1_000726127 antibody / D623_10022376 antibody / H920_20360 antibody / hypothetical protein antibody / I79_017896 antibody / M91_02180 antibody / M959_10865 antibody / MDA_GLEAN10015662 antibody / mitogen and stress-activated protein kinase 1 antibody / mitogen- and stress-activated protein kinase-1 antibody / MSK1 antibody / MSPK1 antibody / N300_00559 antibody / N301_08865 antibody / N302_00067 antibody / N303_08024 antibody / N306_04567 antibody / N307_08443 antibody / N308_06953 antibody / N312_06424 antibody / N320_00446 antibody / N321_03607 antibody / N324_02604 antibody / N326_10342 antibody / N327_13151 antibody / N328_07590 antibody / N329_08096 antibody / N330_05371 antibody / N331_10747 antibody / N332_00298 antibody / N333_06904 antibody / N334_06408 antibody / N335_01622 antibody / N336_10878 antibody / N340_04499 antibody / N341_06419 antibody / nuclear mitogen- and stress-activated protein kinase 1 antibody / PAL_GLEAN10020757 antibody / PANDA_009628 antibody / ribosomal protein S6 kinase / 90kDa / polypeptide 5 antibody / Ribosomal protein S6 kinase alpha-5 antibody / ribosomal protein S6 kinase alpha-5-like protein antibody / ribosomal protein S6 kinase / polypeptide 5 antibody / RLPK antibody / RLSK antibody / RPS6KA5_tv1 antibody / RSKL antibody / RSK-like protein kinase antibody / S6K-alpha-5 antibody / TREES_T100019752 antibody / UY3_02899 antibody / Y1Q_029839 antibody / Y956_05718 antibody / Z169_08798 antibody / zmp:0000000690 antibody
Function Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.
Post-translational Modifications Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain. ; Ubiquitinated.
Cellular Localization Nucleus. Cytoplasm. Note=Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
Tissue Specificity Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

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