SHOPPING BASKET: 0 item(s) - £0.00  

You have no items in your shopping cart.

Anti-Phospho-MSK1 (S360) antibody

[STJ91293] Download PDF Print Data Sheet
Anti-Phospho-MSK1 (S360) antibody
5-7 days

* Required Fields

£0.00
Product name Anti-Phospho-MSK1 (S360) antibody
Short Description Rabbit polyclonal to Phospho-MSK1 (S360).
Description MSK1 is a protein encoded by the RPS6KA5 gene which is approximately 89,8 kDa. MSK1 is localised to the nucleus and cytoplasm. It is involved in activated TLR4 signalling, ERK signalling and RET signalling. It is a serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81. It also contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. MSK1 is widely expressed with high levels in heart, brain and placenta. STJ91293 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MSK1 protein which only binds about S360 when S360 is phosphorylated.
Applications ELISA, IF, IHC-p
Dilution range IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Phospho-MSK1 (S360) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S360.
Protein Name Ribosomal protein S6 kinase alpha-5
S6K-alpha-5
90 kDa ribosomal protein S6 kinase 5
Nuclear mitogen- and stress-activated protein kinase 1
RSK-like protein kinase
RSKL
Immunogen Synthesized peptide derived from human MSK1 around the phosphorylation site of S360.
Immunogen Region 300-380 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 9252
Gene Symbol RPS6KA5
Molecular Weight 90 kDa
Database Links HGNC:10434
OMIM:603607
Alternative Names Anti-Phospho-MSK1 (S360) antibody
Function Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.
Post-translational Modifications Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain. Ubiquitinated.
Cellular Localization Nucleus. Cytoplasm.. Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
Tissue Specificity Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Swiss-Prot Key O75582_HUMAN
Bad Good
Enter the code in the box below:

Ask us a question

No questions yet. Be the first to ask the question!

Tags:

Use spaces to separate tags. Use single quotes (') for phrases.