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Anti-Phospho-p38 (T180/Y182) antibody

[STJ90498] Download PDF Print Data Sheet

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Product name Anti-Phospho-p38 (T180/Y182) antibody
Short Description Rabbit Polyclonal to Phospho-p38 (T180/Y182).
Description p38 is a protein encoded by the MAPK14 gene which is approximately 41,2 kDa. p38 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, toll-like receptor signalling pathways, the VEGF signalling pathway and 4-1BB pathway. This protein falls under the MAP kinase family. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. p38 is expressed in the brain, heart, placenta, pancreas and skeletal muscle. Mutations in the MAPK14 gene may result in patellar tendinitis and lumbosacral lipoma. STJ90498 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous p38 protein which only binds about T180/Y182 when T180/Y182 is phosphorylated.
Applications IHC-p, ELISA, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:5000 /
Specificity Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182.
Protein Name Mitogen-activated protein kinase 14 - MAPK14 - PRKM14 - p38 - Mxi2 - PRKM15 - CSPB1 - CSBP1 - CSBP2 - mitogen-activated protein kinase 14
Immunogen Synthesized peptide derived from human p38 around the phosphorylation site of T180/Y182.
Immunogen Region 120-200 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1432 (Human) / 26416 (Mouse) /
Gene Symbol MAPK14
Molecular Weight 41 kDa
Database Links Human UniProt/Swiss-Prot:Q16539 Mouse UniPort/Swiss-Prot: P47811 Rat UniProt/Swiss-Port: Q56A33 Human Entrez Gene: 1432 Mouse Entrez Gene: 26416 Rat Entrez Gene: Rn.88085
Alternative Names MAPK14 / mitogen-activated protein kinase 14 / AS27_02085 antibody / AS28_11405 antibody / CB1_069740019 antibody / Crk1 antibody / CSAID-binding protein antibody / Csaids binding protein antibody / CSAIDS-binding protein 1 antibody / CSBP antibody / CSBP1 antibody / CSBP2 antibody / cspb1 antibody / cytokine suppressive anti-inflammatory drug binding protein antibody / cytokine suppressive anti-inflammatory drug-binding protein antibody / cytokine suppressive anti-inflammatory drug binding protein 1 antibody / cytokine-supressive anti-inflammatory drug binding protein antibody / D623_10017739 antibody / EGK_14830 antibody / exip antibody / H920_15500 antibody / Hog antibody / hypothetical protein antibody / I79_016605 antibody / M959_14654 antibody / MAPK 14 antibody / mapk14a antibody / MAP kinase 14 antibody / MAP kinase 2 antibody / MAP kinase Mxi2 antibody / MAP kinase p38 alpha antibody / MAPK p38 antibody / MAX-interacting protein 2 antibody / MDA_GLEAN10010694 antibody / mitogen-activated Mitogen-activated protein kinase 2 antibody / Mitogen-activated protein kinase 13 antibody / mitogen activated protein kinase 14 antibody / mitogen-activated protein kinase 14A antibody / Mitogen-activated protein kinase 2 antibody / mitogen-activated protein kinase p38 alpha antibody / MPK2 antibody / mxi2 antibody / N300_03796 antibody / N301_15540 antibody / N302_14711 antibody / N303_14995 antibody / N305_13566 antibody / N306_15633 antibody / N307_09407 antibody / N308_15769 antibody / N309_02157 antibody / N310_02879 antibody / N312_11975 antibody / N322_05034 antibody / N324_05572 antibody / N325_08826 antibody / N328_02959 antibody / N330_12828 antibody / N331_03757 antibody / N333_10851 antibody / N334_02980 antibody / N335_04440 antibody / N336_08941 antibody / N339_09581 antibody / N340_02716 antibody / N341_02075 antibody / p38 antibody / p38a antibody / p38alpha antibody / p38 alpha antibody / p38-alpha antibody / p38alpha Exip antibody / p38 alpha /Mxi2 antibody / p38Hog antibody / p38MAPK antibody / p38 MAPK antibody / p38 MAP kinase antibody / p38 MAP kinase alpha antibody / p38 mitogen-activated kinase antibody / p38 mitogen activated protein kinase antibody / PAL_GLEAN10007048 antibody / PANDA_002221 antibody / PRKM14 antibody / PRKM15 antibody / reactive kinase antibody / RK antibody / sapk2 antibody / sapk2a antibody / stress-activated protein kinase 2A antibody / TREES_T100014798 antibody / tRNA synthetase cofactor p38 antibody / UY3_10532 antibody / Y1Q_016497 antibody / Y956_10892 antibody / Z169_00343 antibody
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'. ; (Microbial infection) Activated by phosphorylation by M.tuberculosis EsxA in T-cells leading to inhibition of IFN-gamma production; phosphorylation is apparent within 15 minute and is inhibited by kinase-specific inhibitors SB203580 and siRNA .
Post-translational Modifications Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 . ; Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3. ; Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Cellular Localization Cytoplasm Nucleus
Tissue Specificity Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.


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