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Anti-Phospho-p70 S6 kinase α (S447) antibody

[STJ90374] Download PDF Print Data Sheet

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Product name Anti-Phospho-p70 S6 kinase α (S447) antibody
Short Description Rabbit Polyclonal to Phospho-p70 S6 kinase α (S447).
Description Phospho-p70 S6 kinase alpha is a protein encoded by the RPS6KB1 gene which is approximately 59,2 kDa. Phospho-p70 S6 kinase alpha is localised to the mitochondrion and cell junctions. It is involved in pathways such as the common cytokine receptor gamma-c family signalling pathway, regulation of lipid metabolism insulin signalling-generic cascades, RET signalling and mTOR signalling. This protein falls under the ribosomal S6 kinase family of serine/threonine kinases. It responds to mTOR signalling and is activated in response to growth factors and nutrients to promote protein synthesis, cell growth, cell cycle progression and cell proliferation. It regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Phospho-p70 S6 kinase alpha is expressed in the cells of the eye, nervous system, muscle, heart and liver. Mutations in the RPS6KB1 gene result in diseases such as tuberous sclerosis which manifests as hamartoma formation in multiple organ systems and glioblastoma multiforme which is a central nervous system tumour of grade IV histological malignancy. STJ90374 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of S447. This primary antibody specifically binds to endogenous p70 S6 kinase α protein which only binds about S447 when S447 is phosphorylated.
Applications IHC-p, ELISA, WB
Dilution range WB 1:500-1:2000 / IHC 1:100-1:300 / ELISA 1:10000 /
Specificity Phospho-p70 S6 kinase α (S447) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S447.
Protein Name Ribosomal protein S6 kinase beta-1 - RPS6KB1 - S6K1 - p70(S6K)-alpha - PS6K - STK14A - ribosomal protein S6 kinase B1
Immunogen Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S447.
Immunogen Region 390-470 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 6198 (Human) / 72508 (Mouse) / 83840 (Rat)
Gene Symbol RPS6KB1
Molecular Weight 70 kDa
Database Links Human UniProt/Swiss-Prot:P23443 Mouse UniPort/Swiss-Prot: Q8BSK8 Rat UniProt/Swiss-Port: P67999 Human Entrez Gene: 6198 Mouse Entrez Gene: 72508 Rat Entrez Gene: Rn.4042
Alternative Names RPS6KB1 / ribosomal protein S6 kinase B1 / 70kDa antibody / 70 kDa ribosomal protein S6 kinase 1 antibody / A306_11714 antibody / AS27_13513 antibody / AS28_07134 antibody / CB1_001084001 antibody / G3 serine /threonine kinase antibody / GW7_08303 antibody / H920_06418 antibody / hypothetical protein antibody / I79_010735 antibody / M91_15751 antibody / M959_13481 antibody / MDA_GLEAN10012250 antibody / N300_11153 antibody / N301_15375 antibody / N302_07699 antibody / N303_14571 antibody / N305_15189 antibody / N306_12110 antibody / N307_14160 antibody / N308_07020 antibody / N309_05752 antibody / N310_08618 antibody / N311_06540 antibody / N312_08740 antibody / N320_10940 antibody / N321_11651 antibody / N322_11038 antibody / N324_11552 antibody / N325_00219 antibody / N326_11241 antibody / N327_09511 antibody / N328_08081 antibody / N329_06062 antibody / N330_08335 antibody / N332_02799 antibody / N333_09739 antibody / N334_00562 antibody / N335_08687 antibody / N336_01007 antibody / N339_08769 antibody / N340_09917 antibody / N341_10126 antibody / p70 /85s6k antibody / p70-alpha antibody / p70 ribosomal S6 kinase alpha antibody / p70-S6K antibody / P70S6K antibody / p70-S6K 1 antibody / P70S6K1 antibody / p70 S6KA antibody / p70 S6K-alpha antibody / p70(S6K)-alpha antibody / p70 S6 kinase alpha antibody / p70 S6 kinase / alpha antibody / PAL_GLEAN10019805 antibody / PANDA_012486 antibody / ps6k antibody / ribosomal protein S6 kinase / 70kDa / polypeptide 1 antibody / ribosomal protein S6 kinase / 70kD / polypeptide 1 antibody / Ribosomal protein S6 kinase beta-1 antibody / ribosomal protein S6 kinase I antibody / ribosomal protein S6 kinase polypeptide 1 antibody / ribosomal protein S6 kinase / polypeptide 1 antibody / rps6ka1 antibody / rps6kb1-A antibody / s6k antibody / s6K1 antibody / S6K-beta-1 antibody / S6 kinase antibody / S6 kinase polypeptide 1 antibody / serine /threonine kinase 14 alpha antibody / serine /threonine-protein kinase 14A antibody / STK14A antibody / TREES_T100015112 antibody / UY3_05008 antibody / Y1Q_030396 antibody / Y956_06538 antibody / Z169_00829 antibody
Function Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.
Post-translational Modifications Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Cellular Localization Cell junction, synapse, synaptosome Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion.; Isoform Alpha I: Nucleus. Cytoplasm.; Isoform Alpha II: Cytoplasm.
Tissue Specificity Widely expressed.
Sequence and Domain Family The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.; The TOS (TOR signaling) motif is essential for activation by mTORC1.

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