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Anti-Phospho-Tau (T205) antibody

[STJ90426] Download PDF Print Data Sheet

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Product name Anti-Phospho-Tau (T205) antibody
Short Description Rabbit Polyclonal to Phospho-Tau (T205).
Description Rabbit Polyclonal to Phospho-Tau (T205).
Applications ELISA, WB
Dilution range WB 1:500-1:2000 / ELISA 1:20000 /
Specificity Phospho-Tau (T205) Polyclonal Antibody detects endogenous levels of Tau protein only when phosphorylated at T205.
Protein Name Microtubule-associated protein tau - MAPT - MTBT1 - tau - PPND - FTDP-17 - TAU - MSTD - MTBT2 - FLJ31424 - MGC138549 - PPP1R103 - DDPAC - MAPTL - microtubule associated protein tau
Immunogen Synthesized peptide derived from human Tau around the phosphorylation site of T205.
Immunogen Region 460-540 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4137 (Human) / 17762 (Mouse) /
Gene Symbol MAPT
Molecular Weight 80 kDa
Database Links Human UniProt/Swiss-Prot:P10636 Mouse UniPort/Swiss-Prot: P10637 Rat UniProt/Swiss-Port: A0JN25 Human Entrez Gene: 4137 Mouse Entrez Gene: 17762 Rat Entrez Gene: Rn.2455
Alternative Names MAPT / microtubule associated protein tau / A306_03881 antibody / AS27_06548 antibody / AS28_04756 antibody / CB1_000313007 antibody / D623_10031080 antibody / DDPAC antibody / ftdp-17 antibody / G protein beta1 /gamma2 subunit-interacting factor 1 antibody / H920_10945 antibody / hypothetical protein antibody / M959_11333 antibody / maptl antibody / mapt microtubule-associated protein tau antibody / MDA_GLEAN10012276 antibody / Microtubule-associated protein tau antibody / microtubule-associated protein tau 464 antibody / microtubule-associated protein tau 611 antibody / microtubule-associated protein tau 688 antibody / microtubule-associated protein tau pseudogene antibody / mstd antibody / Mtapt antibody / mtbt1 antibody / mtbt2 antibody / N300_06227 antibody / N301_11543 antibody / N302_11312 antibody / N303_14149 antibody / N306_08419 antibody / N307_05402 antibody / N308_00512 antibody / N309_04407 antibody / N322_06402 antibody / N326_05233 antibody / N333_11586 antibody / N335_06616 antibody / Neurofibrillary tangle protein antibody / paired helical filament-tau antibody / PAL_GLEAN10004287 antibody / PANDA_017321 antibody / PHF-tau antibody / ppnd antibody / PPP1R103 antibody / protein phosphatase 1 / regulatory subunit 103 antibody / pTau antibody / RNPTAU antibody / TAU antibody / tau-like protein antibody / tau-like protein-2 antibody / Tau microtubule-associated protein antibody / TREES_T100007934 antibody / xtp antibody / Y1Q_004876 antibody / Y956_11244 antibody / Z169_09380 antibody
Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Post-translational Modifications Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1: CDK1, CDK5, GSK3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in the form associated with paired helical filaments (PHF-tau)), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Phosphorylation decreases with age. Phosphorylation within tau/MAP's repeat domain or in flanking regions seems to reduce tau/MAP's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis. Phosphorylation at Ser-548 by GSK3B reduces ability to bind and stabilize microtubules. Phosphorylation at Ser-579 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated at Ser-554, Ser-579, Ser-602, Ser-606 and Ser-669 by PHK. Phosphorylation at Ser-214 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. There is a reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces glycosylation by a factor of 2 and 4 respectively. Phosphorylation on Ser-721 is reduced by about 41.5% by GlcNAcylation on Ser-717. Dephosphorylated at several serine and threonine residues by the serine/threonine phosphatase PPP5C. ; Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur. ; O-glycosylated. O-GlcNAcylation content is around 8.2%. There is reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces O-GlcNAcylation by a factor of 2 and 4 respectively. O-GlcNAcylation on Ser-717 decreases the phosphorylation on Ser-721 by about 41.5%. ; Glycation of PHF-tau, but not normal brain TAU/MAPT. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
Cellular Localization Cytoplasm, cytosol Cell membrane Cytoplasm, cytoskeleton Cell projection, axon Note=Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Tissue Specificity Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Sequence and Domain Family The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.

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