Anti-beta actin antibody




 
Description
Mouse Monoclonal to β-actin.
 


Product Information

Model STJ96941
Host Mouse
Reactivity Chicken, Dog, Hamster, Human, Monkey, Mouse, Rabbit, Rat
Applications WB
Immunogen Synthetic Peptide
Gene ID 60
Dilution range WB 1:2000-5000
Specificity The antibody detects endogenous β-actin protein.
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clone ID 5B7
Note For Research Use Only (RUO).
Protein Name Actin, cytoplasmic 1
Beta-actin
Actin, cytoplasmic 1, N-terminally processed
Clonality Monoclonal
Conjugation Unconjugated
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links HGNC:132
OMIM:102630
Alternative Names ACTB antibody
Actin, cytoplasmic 1 antibody
Beta-actin antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Localization Cytoplasm, cytoskeleton Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Post-translational Modifications ISGylated. ; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). ; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. ; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .


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