Anti-mCherry-Tag Antibody (STJ34373)

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General information

Product name Anti-mCherry-Tag Antibody
Description mCherry (m standing for monomer) is a monomeric fluorophore of approximately 27kDa. First isolated from DsRed of Discosoma, the protein has been incorporated in research to trace molecules and cellular components. Its red fluorescing properties demonstrates its relationship to Green Fluorescent Protein. mCherry is stable and resistant to photobleaching, with peak absorption at 587 nm, as well as a peak emission of 610 nm. 
Host Mouse
Reactivity All
Applications WB, IHC, IF/ICC, IP
Immunogen Full length mCherry-tag protein.
Dilution range WB: 1:3000-1:10000; IHC: 1:200; IF/ICC: 1:200; IP: 1:200
Specificity mCherry-Tag antibody detects endogenous levels of total mCherry-Tag.
Purification Affinity purification
Note For research use only.

Product properties

Clonality Monoclonal
Conjugation Unconjugated
Isotype IgG1
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Concentration 1 mg/ml
Storage Instruction Store at -20°C. Avoid repeated freeze/thaw cycles.

Target

Product images-applications

Image shows western blot analysis of STJ34373 anti-mCherry-Tag Antibody diluted 1:500 with 5 min exposure time. Hela cell line tested at 24, 48 & 72 hours after mCherry transfection.

Image shows paraffin-embedded immunohistochemistry performed using anti-mCherry-Tag Antibody.

A549 cells transfected with mCherry vector were stained with anti-mcherry-Tag Antibody (green). Overlap of green and red (natural mCherry exposure) demonstrates mCherry specificity.

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We submitted our mCherry-Tag antibody (ST34373) to an independent facility for validation testing. The testing, carried out by Science Exchange, confirmed that STJ34373 demonstrated specificity for mCherry under Western Blot analysis, Immunohistochemistry and Immunofluorescence.

Independent Validation Review: mCherry Tag Monoclonal Antibody (STJ34373)

Independent Validation Review for mCherry Monoclonal Antibody - Immunohistochemistry Data
 

Independent Validation Review for mCherry Monoclonal Antibody - Immunofluorescence Data
 

Independent Validation Review for mCherry Monoclonal Antibody - Western Blot Data
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