General information

Product name Anti-Caspase-3 antibody
Description Rabbit Polyclonal to Caspase-3.
Host Rabbit
Reactivity Human, Monkey
Applications WB, ELISA
Immunogen Synthesized peptide derived from the N-terminal region of human Caspase-3 at AA range: 20-100.
Immunogen Region 20-100aa
Gene ID 836
Dilution range WB 1:500-1:2000; ELISA 1:40000;
Specificity Caspase-3 Polyclonal Antibody detects endogenous levels of Caspase-3 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note For research use only.
Protein Name Caspase-3

Product properties

Clonality Polyclonal
Conjugation Unconjugated
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Molecular Weight 35 kDa
Concentration 1 mg/ml
Storage Instruction Store at -20°C. Avoid repeated freeze/thaw cycles.

Target

Database Links Genecards: 836 Swiss-Prot: P42574 HGNC: 1504 Entrez gene: 836
Alternative names
CASP3 / caspase 3 / AC-3 antibody, Apopain antibody, CASP-3 antibody, casp3 protein antibody, Caspase-3 antibody, caspase 3A antibody, caspase 3, apoptosis-related cysteine peptidase antibody, caspase 3, apoptosis related cysteine protease antibody, caspase 3, apoptosis-related cysteine protease antibody, Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease) antibody, Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease) antibody, caspase 3-like antibody, Caspase-3-like protein antibody, caspase-3-like protein 1 antibody, caspase-3 preproprotein antibody, CB1_000998003 antibody, CC3 antibody, cpp32 antibody, CPP-32 antibody, CPP32B antibody, CPP32beta antibody, Cysteine protease CPP32 antibody, GW7_02720 antibody, H920_14272 antibody, hypothetical protein antibody, IRP antibody, Lice antibody, MDA_GLEAN10021003 antibody, mldy antibody, PAL_GLEAN10021656 antibody, PANDA_001909 antibody, PARP cleavage protease antibody, procaspase3 antibody, Protein Yama antibody, SCA-1 antibody, SREBP cleavage activity 1 antibody, UY3_01982 antibody, xCPP32 antibody, Y1Q_030565 antibody, Yama antibody, yama protein antibody
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. / Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. / Inhibited by isatin sulfonamides.
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Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence and Domain Family Belongs to the peptidase C14A family.
Post-translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. / S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Cellular Localization Cytoplasm

Product images-applications

Western Blot (WB) analysis of specific cells using Caspase-3 Polyclonal Antibody.

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Review by J. Huang, August 2016.

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