Anti-Beta Actin Antibody (STJ96941)

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General information

Product name Anti-Beta Actin Antibody
Description Beta actin is a protein encoded by the ACTB gene which is approximately 41,7 kDa. Beta actin is localised to the cytoplasm. It is involved in pathways such as blood-brain barrier and immune cell transmigration, regulation of actin cytoskeleton by Rho GTPases and development Slit-Robo signalling. This protein falls under the actin family. There are 3 main groups of actin isoforms in vertebrates namely, alpha, beta and gamma actins. Beta actin is a highly conserved proteins that is involved in various types of internal cell motility and are the major constituent of microfilaments. Beta actin is ubiquitously expressed in all eukaryotic cells. Mutations in the ACTB gene result in a form of juvenile-onset dystonia, which is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Baraitser-Winter syndrome 1 is another disease that can occur due to mutations. This is rare developmental disorder that causes postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. STJ96941 was developed from clone 5B7. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody binds endogenous beta actin.
Host Mouse
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit
Applications WB
Immunogen Synthetic Peptide
Gene ID 60
Dilution range WB 1:2000-5000
Specificity The antibody detects endogenous Beta Actin protein.
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clone ID 5B7
Note For research use only.
Protein Name Actin cytoplasmic 1

Product properties

Clonality Monoclonal
Conjugation Unconjugated
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C. Avoid repeated freeze/thaw cycles.

Target

Database Links Genecards: 60 Swiss-Prot: P60709 HGNC: 132 Entrez gene: 60
Alternative names
ACTB / actin, beta / act-2 antibody, ACT-5 antibody, β-actin, ACTA1 antibody, actba antibody, actin antibody, actin, alpha 1, skeletal muscle antibody, actin beta antibody, actin, beta, cytoplasmic antibody, Actin, cytoplasmic 1 antibody, Actin, cytoplasmic type 5 antibody, Actx antibody, Anapl_00512 antibody, AS27_12274 antibody, AS28_05902 antibody, A-X actin-like protein antibody, Bact antibody, Bact; actin antibody, B-actin antibody, beta actin antibody, Beta-actin antibody, beta actin1 antibody, Beta-actin A antibody, beta cytoskeletal actin antibody, BRWS1 antibody, cytoplasmic 1 antibody, cytoplasmic actin OlCA1 antibody, cytoplasmic beta actin antibody, cytoplasmic beta-actin antibody, cytoskeletal beta actin antibody, D623_10011024 antibody, gamma non-muscle actin antibody, GW7_16883 antibody, H920_13304 antibody, hypothetical protein antibody, M91_16383 antibody, M959_04960 antibody, MDA_GLEAN10021467 antibody, melanoma X-actin antibody, N300_09809 antibody, N301_15340 antibody, N302_11832 antibody, N303_00828 antibody, N305_14864 antibody, N306_14056 antibody, N307_07714 antibody, N308_12235 antibody, N309_01588 antibody, N312_00100 antibody, olCA1 antibody, PAL_GLEAN10018968 antibody, PANDA_004593 antibody, PS1TP5-binding protein 1 antibody, ps1tp5bp1 antibody, putative beta-actin antibody, TREES_T100021367 antibody, UY3_09776 antibody, Y1Q_025431 antibody, Y956_00599 antibody, Z169_07136 antibody
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Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Sequence and Domain Family Belongs to the actin family.
Post-translational Modifications ISGylated. / Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). / Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. / (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .
Cellular Localization Cytoplasm > cytoskeleton

Product images-applications

Western Blot (WB) analysis of HeLa, with Anti-Beta Actin Antibody diluted at 1:3000.

Western blot analysis of normal NPC (lane 1) and TRF2 Knockdown (lane 2) NPCs. Anti-Beta Actin Antibody was diluted at 1:1000.

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Review by Viva Biotech Ltd. 

Review by Sitti, March 2017.

Western Blot Antibody Customer Review for Anti-β-actin Antibody (STJ96941)
 
Review by Marie Davies, March 2017.

 

Western Blot Customer Review Anti-β-actin Antibody (STJ96941) 
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