• Western blot analysis of recombinant SARS-COV-2 N Protein, using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (STJ11101210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-2019-nCoV antibody [S0MR] (STJ11101210)

SKU:
STJ11101210

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Host: Rabbit
Applications: WB/IF
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SARS-COV-2 N Protein is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at 4°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Recombinant fusion protein of SARS-CoV-2 Nucleoprotein Full length ( P59595 ).
Immunogen Sequence: MSDNGPQSNQRSAPRITFGG PTDSTDNNQNGGRNGARPKQ RRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSG PDDQIGYYRRATRRVRGGDG KMKELSPRWYFYYLGTGPEA SLPYGANKEGIVWVATEGAL NTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRGG SQASSRSSSRSRGNSRNSTP GSSRGNSPARMASGGGETAL ALLLLDRLNQLESKVSGKG
Background This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

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