• Western blot analysis of extracts of various cell lines, using GABA transaminase (GABA transaminase (ABAT) ) Polyclonal Antibody (STJ27252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of HepG2 cells, using GABA transaminase (GABA transaminase (ABAT) ) Polyclonal Antibody (STJ27252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of mouse brain using GABA transaminase (GABA transaminase (ABAT) ) rabbit polyclonal antibody (STJ27252) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ABAT antibody (29-300) (STJ27252)

SKU:
STJ27252

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABAT (29-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABAT
Gene ID: 18
Uniprot ID: GABT_HUMAN
Immunogen Region: 29-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human GABA transaminase (GABA transaminase (ABAT) ) (NP_001120920.1).
Immunogen Sequence: SQAAAKVDVEFDYDGPLMKT EVPGPRSQELMKQLNIIQNA EAVHFFCNYEESRGNYLVDV DGNRMLDLYSQISSVPIGYS HPALLKLIQQPQNASMFVNR PALGILPPENFVEKLRQSLL SVAPKGMSQLITMACGSCSN ENALKTIFMWYRSKERGQRG FSQEELETCMINQAPGCPDY SILSFMGAFHGRTMGCLATT HSKAIHKIDIPSFDWPIAPF PRLKYPLEEFVKENQQEEA
Tissue Specificity Liver > pancreas > brain > kidney > heart > placenta.
Function Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Protein Name 4-Aminobutyrate Aminotransferase - Mitochondrial
(S-3-Amino-2-Methylpropionate Transaminase
Gaba Aminotransferase
Gaba-At
Gamma-Amino-N-Butyrate Transaminase
Gaba Transaminase
Gaba-T
L-Aibat
Database Links Reactome: R-HSA-916853
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-4-Aminobutyrate Aminotransferase - Mitochondrial antibody
Anti-(S-3-Amino-2-Methylpropionate Transaminase antibody
Anti-Gaba Aminotransferase antibody
Anti-Gaba-At antibody
Anti-Gamma-Amino-N-Butyrate Transaminase antibody
Anti-Gaba Transaminase antibody
Anti-Gaba-T antibody
Anti-L-Aibat antibody
Anti-ABAT antibody
Anti-GABAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance