• Western blot analysis of extracts of various cell lines, using ACE2 antibody (STJ114610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded rat lung using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat testis using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse testis using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse kidney using ACE2 rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACE2 antibody (18-740) (STJ114610)

SKU:
STJ114610

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACE2 (18-740) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACE2
Gene ID: 59272
Uniprot ID: ACE2_HUMAN
Immunogen Region: 18-740
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 18-740 of human ACE2 (NP_068576.1).
Immunogen Sequence: QSTIEEQAKTFLDKFNHEAE DLFYQSSLASWNYNTNITEE NVQNMNNAGDKWSAFLKEQS TLAQMYPLQEIQNLTVKLQL QALQQNGSSVLSEDKSKRLN TILNTMSTIYSTGKVCNPDN PQECLLLEPGLNEIMANSLD YNERLWAWESWRSEVGKQLR PLYEEYVVLKNEMARANHYE DYGDYWRGDYEVNGVDGYDY SRGQLIEDVEHTFEEIKPLY EHLHAYVRAKLMNAYPSYI
Tissue Specificity Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level). Expressed in nasal epithelial cells (at protein level). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells. Coexpressed with TMPRSS4 within mature enterocytes. Isoform 2: Expressed in nasal and bronchial epithelial cells (at protein level).
Post Translational Modifications N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains.
Function Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Protein Name Angiotensin-Converting Enzyme 2
Angiotensin-Converting Enzyme Homolog
Aceh
Angiotensin-Converting Enzyme-Related Carboxypeptidase
Ace-Related Carboxypeptidase
Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-9678110
Reactome: R-HSA-9679191
Reactome: R-HSA-9694614
Reactome: R-HSA-9733458
Cellular Localisation Processed Angiotensin-Converting Enzyme 2: Secreted
Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Cell Projection
Cilium
Apical Cell Membrane
Detected In Both Cell Membrane And Cytoplasm In Neurons
Isoform 2: Apical Cell Membrane
Alternative Antibody Names Anti-Angiotensin-Converting Enzyme 2 antibody
Anti-Angiotensin-Converting Enzyme Homolog antibody
Anti-Aceh antibody
Anti-Angiotensin-Converting Enzyme-Related Carboxypeptidase antibody
Anti-Ace-Related Carboxypeptidase antibody
Anti-Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 antibody
Anti-ACE2 antibody
Anti-UNQ868 antibody
Anti-PRO1885 antibody

Information sourced from Uniprot.org

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