• Western blot analysis of extracts of various cell lines, using Aconitase 2 antibody (STJ22487) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of Rat kidney, using Aconitase 2 antibody (STJ22487) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded Rat heart using Aconitase 2 antibody (STJ22487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil using Aconitase 2 antibody (STJ22487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Aconitase 2 antibody (STJ22487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Aconitase 2 antibody (STJ22487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACO2 antibody (501-780) (STJ22487)

SKU:
STJ22487

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACO2 (501-780) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACO2
Gene ID: 50
Uniprot ID: ACON_HUMAN
Immunogen Region: 501-780
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human Aconitase 2 (Aconitase 2 (ACO2) ) (NP_001089.1).
Immunogen Sequence: IAGTLKFNPETDYLTGTDGK KFRLEAPDADELPKGEFDPG QDTYQHPPKDSSGQHVDVSP TSQRLQLLEPFDKWDGKDLE DLQILIKVKGKCTTDHISAA GPWLKFRGHLDNISNNLLIG AINIENGKANSVRNAVTQEF GPVPDTARYYKKHGIRWVVI GDENYGEGSSREHAALEPRH LGGRAIITKSFARIHETNLK KQGLLPLTFADPADYNKIHP VDKLTIQGLKDFTPGKPLK
Post Translational Modifications Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers.
Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Protein Name Aconitate Hydratase - Mitochondrial
Aconitase
Citrate Hydro-Lyase
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Aconitate Hydratase - Mitochondrial antibody
Anti-Aconitase antibody
Anti-Citrate Hydro-Lyase antibody
Anti-ACO2 antibody

Information sourced from Uniprot.org

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