• Western blot analysis of extracts of various cell lines, using ACTR1A antibody (STJ28598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ACTR1A antibody (187-376) (STJ28598)

SKU:
STJ28598

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACTR1A (187-376) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTR1A
Gene ID: 10121
Uniprot ID: ACTZ_HUMAN
Immunogen Region: 187-376
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 187-376 of human ACTR1A (NP_005727.1).
Immunogen Sequence: GRDVSRFLRLYLRKEGYDFH SSSEFEIVKAIKERACYLSI NPQKDETLETEKAQYYLPDG STIEIGPSRFRAPELLFRPD LIGEESEGIHEVLVFAIQKS DMDLRRTLFSNIVLSGGSTL FKGFGDRLLSEVKKLAPKDV KIRISAPQERLYSTWIGGSI LASLDTFKKMWVSKKEYEED GARSIHRKTF
Function Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.
Protein Name Alpha-Centractin
Centractin
Arp1
Actin-Rpv
Centrosome-Associated Actin Homolog
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-2565942
Reactome: R-HSA-3371497
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Cortex
Alternative Antibody Names Anti-Alpha-Centractin antibody
Anti-Centractin antibody
Anti-Arp1 antibody
Anti-Actin-Rpv antibody
Anti-Centrosome-Associated Actin Homolog antibody
Anti-ACTR1A antibody
Anti-CTRN1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance