• Western blot analysis of lysates from HepG2 cells, using ADARB1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using ADAR2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ADARB1 antibody (481-530 aa) (STJ91484)

SKU:
STJ91484

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Double-stranded RNA-specific editase 1 (481-530 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADARB1
Gene ID: 104
Uniprot ID: RED1_HUMAN
Immunogen Region: 481-530 aa
Specificity: ADAR2 Polyclonal Antibody detects endogenous levels of ADAR2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ADARB1 at the amino acid range 481-530
Function Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins.pre-mRNA splicing by altering splice site recognition sequences.RNA stability by changing sequences involved in nuclease recognition.genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication.and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis. Isoform 1: Has a lower catalytic activity than isoform 2. Isoform 2: Has a higher catalytic activity than isoform 1.
Protein Name Double-Stranded Rna-Specific Editase 1
Rna-Editing Deaminase 1
Rna-Editing Enzyme 1
Dsrna Adenosine Deaminase
Database Links Reactome: R-HSA-75102
Reactome: R-HSA-77042
Cellular Localisation Nucleus
Nucleolus
Shuttles Between Nucleoli And The Nucleoplasm
Isoform 1: Nucleus
Isoform 2: Nucleus
Alternative Antibody Names Anti-Double-Stranded Rna-Specific Editase 1 antibody
Anti-Rna-Editing Deaminase 1 antibody
Anti-Rna-Editing Enzyme 1 antibody
Anti-Dsrna Adenosine Deaminase antibody
Anti-ADARB1 antibody
Anti-ADAR2 antibody
Anti-DRADA2 antibody
Anti-RED1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance