• Western blot analysis of extracts of rat liver, using ADH1B antibody (STJ22517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADH1B antibody (206-375) (STJ22517)

SKU:
STJ22517

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADH1B (206-375) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADH1B
Gene ID: 125
Uniprot ID: ADH1B_HUMAN
Immunogen Region: 206-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-375 of human ADH1B (NP_000659.2).
Immunogen Sequence: LSAVMGCKAAGAARIIAVDI NKDKFAKAKELGATECINPQ DYKKPIQEVLKEMTDGGVDF SFEVIGRLDTMMASLLCCHE ACGTSVIVGVPPASQNLSIN PMLLLTGRTWKGAVYGGFKS KEGIPKLVADFMAKKFSLDA LITHVLPFEKINEGFDLLHS GKSIRTVLTF
Function Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal. Catalyzes in the oxidative direction with higher efficiency. Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal.
Protein Name All-Trans-Retinol Dehydrogenase Nad(+ Adh1b
Alcohol Dehydrogenase 1b
Alcohol Dehydrogenase Subunit Beta
Database Links Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-All-Trans-Retinol Dehydrogenase Nad(+ Adh1b antibody
Anti-Alcohol Dehydrogenase 1b antibody
Anti-Alcohol Dehydrogenase Subunit Beta antibody
Anti-ADH1B antibody
Anti-ADH2 antibody

Information sourced from Uniprot.org

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