• Immunofluorescence analysis of NIH/3T3 cells using ADORA2A antibody (STJ22527) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADORA2A antibody (STJ22527) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADORA2A antibody (STJ22527) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of SH-SY5Y cells, using ADORA2A antibody (STJ22527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ADORA2A antibody (300-400) (STJ22527)

SKU:
STJ22527

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADORA2A (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADORA2A
Gene ID: 135
Uniprot ID: AA2AR_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human ADORA2A (NP_000666.2).
Immunogen Sequence: RKIIRSHVLRQQEPFKAAGT SARVLAAHGSDGEQVSLRLN GHPPGVWANGSAPHPERRPN GYALGLVSGGSAQESQGNTG LPDVELLSHELKGVCPEPPG L
Post Translational Modifications Ubiquitinated. Deubiquitinated by USP4.leading to stabilization and expression at the cell surface.
Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Protein Name Adenosine Receptor A2a
Database Links Reactome: R-HSA-187024
Reactome: R-HSA-417973
Reactome: R-HSA-418555
Reactome: R-HSA-5683826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Colocalizes With Gas2l2 At Neuronal Processes
Alternative Antibody Names Anti-Adenosine Receptor A2a antibody
Anti-ADORA2A antibody
Anti-ADORA2 antibody

Information sourced from Uniprot.org

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