• Western blot analysis of extracts of various cell lines, using ALDH3B1 antibody (STJ112450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ALDH3B1 antibody (1-80) (STJ112450)

SKU:
STJ112450

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH3B1 (1-80) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH3B1
Gene ID: 221
Uniprot ID: AL3B1_HUMAN
Immunogen Region: 1-80
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human ALDH3B1 (NP_001025181.1).
Immunogen Sequence: MDPLGDTLRRLREAFHAGRT RPAEFRAAQLQGLGRFLQEN KQLLHDALAQDLHKATQLDS AFIRKEPFGLVLIIAPWNYP
Tissue Specificity Highest expression in kidney and lung.
Post Translational Modifications Dually lipidated in the C-terminus.prenylation occurs prior to, and is a prerequisite for palmitoylation. It is also required for activity towards long-chain substrates.
Function Oxidizes medium and long chain saturated and unsaturated aldehydes. Metabolizes also benzaldehyde. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde. May not metabolize short chain aldehydes. Can use both NADP(+) and NAD(+) as electron acceptor. May have a protective role against the cytotoxicity induced by lipid peroxidation.
Protein Name Aldehyde Dehydrogenase Family 3 Member B1
Aldehyde Dehydrogenase 7
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Lipid-Anchor
Primarily In The Plasma Membrane As Well As In Some Punctate Structures In The Cytoplasm
Alternative Antibody Names Anti-Aldehyde Dehydrogenase Family 3 Member B1 antibody
Anti-Aldehyde Dehydrogenase 7 antibody
Anti-ALDH3B1 antibody
Anti-ALDH7 antibody

Information sourced from Uniprot.org

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