• Western blot analysis of extracts of various cell lines, using ALDOB antibody (STJ22585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using ALDOB rabbit polyclonal antibody (STJ22585) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using ALDOB rabbit polyclonal antibody (STJ22585) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDOB antibody (1-200) (STJ22585)

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STJ22585

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDOB (1-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDOB
Gene ID: 229
Uniprot ID: ALDOB_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALDOB (NP_000026.2).
Immunogen Sequence: MAHRFPALTQEQKKELSEIA QSIVANGKGILAADESVGTM GNRLQRIKVENTEENRRQFR EILFSVDSSINQSIGGVILF HETLYQKDSQGKLFRNILKE KGIVVGIKLDQGGAPLAGTN KETTIQGLDGLSERCAQYKK DGVDFGKWRAVLRIADQCPS SLAIQENANALARYASICQQ NGLVPIVEPEVIPDGDHDLE
Function Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde. Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check.
Protein Name Fructose-Bisphosphate Aldolase B
Liver-Type Aldolase
Database Links Reactome: R-HSA-5657560
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Reactome: R-HSA-70350
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriolar Satellite
Alternative Antibody Names Anti-Fructose-Bisphosphate Aldolase B antibody
Anti-Liver-Type Aldolase antibody
Anti-ALDOB antibody
Anti-ALDB antibody

Information sourced from Uniprot.org

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