• Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
  • Immunofluorescence analysis of Rat-spinal-cord tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1 Mu M, 18 hr; +), using Acetyl-a-tubulin (Lys40) Mouse mAb 1:2000.
  • Immunofluorescence analysis of Mouse-lung tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-liver-cancer tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Acetyl-TUBA1B-4A8 antibody [4A8] (STJ97537)

SKU:
STJ97537

Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Rat/Mouse/D.melanogaster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Acetyl-Tubulin alpha-1B chain-4A8 is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 4A8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
IHC 1:50-100
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBA1B
Gene ID: 10376
Uniprot ID: TBA1B_HUMAN
Specificity: Alpha-tubulin (Acetyl Lys40) protein detects endogenous levels of Alpha-tubulin (Acetyl Lys40)
Immunogen: Synthetic Peptide of Alpha-tubulin (Acetyl Lys40)
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name Tubulin Alpha-1b Chain
Alpha-Tubulin Ubiquitous
Tubulin K-Alpha-1
Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-3371497
Reactome: R-HSA-380320
Reactome: R-HSA-389960
Reactome: R-HSA-389977
Reactome: R-HSA-437239
Reactome: R-HSA-5610787
Reactome: R-HSA-5617833
Reactome: R-HSA-5620924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8852276
Reactome: R-HSA-8955332
Reactome: R-HSA-9013407
Reactome: R-HSA-9609690
Reactome: R-HSA-9609736
Reactome: R-HSA-9619483
Reactome: R-HSA-9646399
Reactome: R-HSA-9648025
Reactome: R-HSA-9668328
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tubulin Alpha-1b Chain antibody
Anti-Alpha-Tubulin Ubiquitous antibody
Anti-Tubulin K-Alpha-1 antibody
Anti-Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain antibody
Anti-TUBA1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance