• Western blot analysis of extracts of various cell lines, using APOBEC3F antibody (STJ22650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded rat ovary using APOBEC3F antibody (STJ22650) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human lung cancer using APOBEC3F antibody (STJ22650) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse lung using APOBEC3F antibody (STJ22650) at dilution of 1:100 (40x lens).

Anti-APOBEC3F antibody (1-65) (STJ22650)

SKU:
STJ22650

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOBEC3F (1-65) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC3F
Gene ID: 200316
Uniprot ID: ABC3F_HUMAN
Immunogen Region: 1-65
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human APOBEC3F (NP_660341.2).
Immunogen Sequence: MKPHFRNTVERMYRDTFSYN FYNRPILSRRNTVWLCYEVK TKGPSRPRLDAKIFRGQVYS QPEHH
Tissue Specificity Widely expressed. Highly expressed in ovary.
Function DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against viruse such as HIV-1 or HIV-2. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Protein Name Dna Dc->Du-Editing Enzyme Apobec-3f
Apolipoprotein B Mrna-Editing Enzyme Catalytic Polypeptide-Like 3f
A3f
Cellular Localisation Cytoplasm
P-Body
Alternative Antibody Names Anti-Dna Dc->Du-Editing Enzyme Apobec-3f antibody
Anti-Apolipoprotein B Mrna-Editing Enzyme Catalytic Polypeptide-Like 3f antibody
Anti-A3f antibody
Anti-APOBEC3F antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance