• Western blot analysis of lysates from 22Rv1 cells, using APOH Rabbit polyclonal antibody (STJ22654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-APOH antibody (21-345) (STJ22654)

SKU:
STJ22654

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOH (21-345) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOH
Gene ID: 350
Uniprot ID: APOH_HUMAN
Immunogen Region: 21-345
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-345 of human APOH (NP_000033.2).
Immunogen Sequence: RTCPKPDDLPFSTVVPLKTF YEPGEEITYSCKPGYVSRGG MRKFICPLTGLWPINTLKCT PRVCPFAGILENGAVRYTTF EYPNTISFSCNTGFYLNGAD SAKCTEEGKWSPELPVCAPI ICPPPSIPTFATLRVYKPSA GNNSLYRDTAVFECLPQHAM FGNDTITCTTHGNWTKLPEC REVKCPFPSRPDNGFVNYPA KPTLYYKDKATFGCHDGYSL DGPEEIECTKLGNWSAMPS
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
Function Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Protein Name Beta-2-Glycoprotein 1
Apc Inhibitor
Activated Protein C-Binding Protein
Anticardiolipin Cofactor
Apolipoprotein H
Apo-H
Beta-2-Glycoprotein I
B2gpi
Beta(2gpi
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Alternative Antibody Names Anti-Beta-2-Glycoprotein 1 antibody
Anti-Apc Inhibitor antibody
Anti-Activated Protein C-Binding Protein antibody
Anti-Anticardiolipin Cofactor antibody
Anti-Apolipoprotein H antibody
Anti-Apo-H antibody
Anti-Beta-2-Glycoprotein I antibody
Anti-B2gpi antibody
Anti-Beta(2gpi antibody
Anti-APOH antibody
Anti-B2G1 antibody

Information sourced from Uniprot.org

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