• Western blot analysis of extracts of various cell lines, using p16-ARC/ARC16/p16-ARC/ARC16/ARPC5 antibody (STJ111306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using p16-ARC/ARC16/p16-ARC/ARC16/ARPC5 antibody (STJ111306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARPC5 antibody (1-154) (STJ111306)

SKU:
STJ111306

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARPC5 (1-154) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARPC5
Gene ID: 10092
Uniprot ID: ARPC5_HUMAN
Immunogen Region: 1-154
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human p16-ARC/ARC16/p16-ARC/ARC16/ARPC5 (NP_001257368.1).
Immunogen Sequence: MSKNTVSSARFRKVDVDEYD ENKFVDEEDGGDGQAGPDEG EVDSCLRHSITGNMTAALQA ALKNPPINTKSQAVKDRAGS IVLKVLISFKANDIEKAVQS LDKNGVDLLMKYIYKGFESP SDNSSAMLLQWHEKALAAGG VGSIVRVLTARKTV
Post Translational Modifications Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.
Function Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Protein Name Actin-Related Protein 2/3 Complex Subunit 5
Arp2/3 Complex 16 Kda Subunit
P16-Arc
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-6798695
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Nucleus
Alternative Antibody Names Anti-Actin-Related Protein 2/3 Complex Subunit 5 antibody
Anti-Arp2/3 Complex 16 Kda Subunit antibody
Anti-P16-Arc antibody
Anti-ARPC5 antibody
Anti-ARC16 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance