• Immunofluorescence analysis of U-2 OS cells using ART1 Rabbit polyclonal antibody (STJ112142) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ART1 Rabbit polyclonal antibody (STJ112142) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ART1 in paraffin-embedded human lung cancer using ART1 Rabbit polyclonal antibody (STJ112142) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ART1 Rabbit polyclonal antibody (STJ112142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ART1 antibody (23-295) (STJ112142)

SKU:
STJ112142

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ART1 (23-295) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ART1
Gene ID: 417
Uniprot ID: NAR1_HUMAN
Immunogen Region: 23-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-295 of human ART1 (NP_004305.2).
Immunogen Sequence: QSHPITRRDLFSQEIQLDMA LASFDDQYAGCAAAMTAALP DLNHTEFQANQVYADSWTLA SSQWQERQARWPEWSLSPTR PSPPPLGFRDEHGVALLAYT ANSPLHKEFNAAVREAGRSR AHYLHHFSFKTLHFLLTEAL QLLGSGQRPPRCHQVFRGVH GLRFRPAGPRATVRLGGFAS ASLKHVAAQQFGEDTFFGIW TCLGAPIKGYSFFPGEEEVL IPPFETFQVINASRLAQGP
Function Has ADP-ribosyltransferase activity toward GLP1R.
Protein Name Gpi-Linked Nad(P(+--Arginine Adp-Ribosyltransferase 1
Adp-Ribosyltransferase C2 And C3 Toxin-Like 1
Artc1
Mono(Adp-Ribosyltransferase 1
Cd Antigen Cd296
Database Links Reactome: R-HSA-1462054
Cellular Localisation Sarcoplasmic Reticulum Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Gpi-Linked Nad(P(+--Arginine Adp-Ribosyltransferase 1 antibody
Anti-Adp-Ribosyltransferase C2 And C3 Toxin-Like 1 antibody
Anti-Artc1 antibody
Anti-Mono(Adp-Ribosyltransferase 1 antibody
Anti-Cd Antigen Cd296 antibody
Anti-ART1 antibody

Information sourced from Uniprot.org

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