• Western blot analysis of extracts of various cell lines, using ATP6V1A antibody (STJ116906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ATP6V1A antibody (500-600) (STJ116906)

SKU:
STJ116906

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1A (500-600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1A
Gene ID: 523
Uniprot ID: VATA_HUMAN
Immunogen Region: 500-600
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
Immunogen Sequence: SLAETDKITLEVAKLIKDDF LQQNGYTPYDRFCPFYKTVG MLSNMIAFYDMARRAVETTA QSDNKITWSIIREHMGDILY KLSSMKFKDPLKDGEAKIKS D
Tissue Specificity High expression in the skin.
Post Translational Modifications Phosphorylation at Ser-384 by AMPK down-regulates its enzyme activity.
Function Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity. (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.
Protein Name V-Type Proton Atpase Catalytic Subunit A
V-Atpase Subunit A
V-Atpase 69 Kda Subunit
Vacuolar Atpase Isoform Va68
Vacuolar Proton Pump Subunit Alpha
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Cellular Localisation Cytoplasm
Cytosol
Cytoplasmic Vesicle
Secretory Vesicle
Clathrin-Coated Vesicle Membrane
Peripheral Membrane Protein
Lysosome
Co-Localizes With Wfs1 In The Secretory Granules In Neuroblastoma Cell Lines
Alternative Antibody Names Anti-V-Type Proton Atpase Catalytic Subunit A antibody
Anti-V-Atpase Subunit A antibody
Anti-V-Atpase 69 Kda Subunit antibody
Anti-Vacuolar Atpase Isoform Va68 antibody
Anti-Vacuolar Proton Pump Subunit Alpha antibody
Anti-ATP6V1A antibody
Anti-ATP6A1 antibody
Anti-ATP6V1A1 antibody
Anti-VPP2 antibody

Information sourced from Uniprot.org

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