• Western blot analysis of extracts of various cell lines, using ATP6V1D antibody (STJ114806) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATP6V1D antibody (1-247) (STJ114806)

SKU:
STJ114806

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1D (1-247) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1D
Gene ID: 51382
Uniprot ID: VATD_HUMAN
Immunogen Region: 1-247
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human ATP6V1D (NP_057078.1).
Immunogen Sequence: MSGKDRIEIFPSRMAQTIMK ARLKGAQTGRNLLKKKSDAL TLRFRQILKKIIETKMLMGE VMREAAFSLAEAKFTAGDFS TTVIQNVNKAQVKIRAKKDN VAGVTLPVFEHYHEGTDSYE LTGLARGGEQLAKLKRNYAK AVELLVELASLQTSFVTLDE AIKITNRRVNAIEHVIIPRI ERTLAYIITELDEREREEFY RLKKIQEKKKILKEKSEKDL EQRRAAGEVLEPANLLAEE
Function Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
Protein Name V-Type Proton Atpase Subunit D
V-Atpase Subunit D
V-Atpase 28 Kda Accessory Protein
Vacuolar Proton Pump Subunit D
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-6798695
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Cellular Localisation Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Clathrin-Coated Vesicle Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Projection
Cilium
Localizes To Centrosome And The Base Of The Cilium
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit D antibody
Anti-V-Atpase Subunit D antibody
Anti-V-Atpase 28 Kda Accessory Protein antibody
Anti-Vacuolar Proton Pump Subunit D antibody
Anti-ATP6V1D antibody
Anti-ATP6M antibody
Anti-VATD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance