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Anti-β-actin antibody

[STJ97089] Download PDF Print Data Sheet
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Product name Anti-β-actin antibody
Description Rabbit polyclonal to β-actin.
Applications IHC, WB
Dilution range WB 1:3000
IHC 1:200
Specificity The antibody detects endogenous β-actin protein.
Protein Name Actin, cytoplasmic 1
Beta-actin
Actin, cytoplasmic 1, N-terminally processed
Immunogen Recombinant Protein
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Chicken, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 60
Gene Symbol ACTB
Database Links HGNC:132
OMIM:102630
Alternative Names β-actin
Actin, cytoplasmic 1 antibody
Beta-actin antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational Modifications ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .
Cellular Localization Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Swiss-Prot Key P60709_HUMAN
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I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 13:40:00
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Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 13:43:00

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