• Western blot analysis of extracts of various cell lines, using Brat1 antibody (STJ114667) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BRAT1 antibody (652-821) (STJ114667)

SKU:
STJ114667

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRAT1 (652-821) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRAT1
Gene ID: 221927
Uniprot ID: BRAT1_HUMAN
Immunogen Region: 652-821
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 652-821 of human BRAT1 (NP_689956.2).
Immunogen Sequence: AQGLELALVFLGQTLGPPRT HCPYAVALPEVAPAQPLTEA LRALCHVGLFDFAFCALFDC DRPVAQKSCDLLLFLRDKIA SYSSLREARGSPNTASAEAT LPRWRAGEQAQPPGDQEPEA VLAMLRSLDLEGLRSTLAES SDHVEKSPQSLLQDMLATGG FLQGDEADCY
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Ubiquitinated by NEDD4, NEDD4L and ITCH.mono- and polyubiquitinated forms are detected.
Function Involved in DNA damage response.activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. Plays a role in regulating mitochondrial function and cell proliferation. Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors.
Protein Name Brca1-Associated Atm Activator 1
Brca1-Associated Protein Required For Atm Activation Protein 1
Cellular Localisation Nucleus
Cytoplasm
Present At Double Strand Breaks (Dsbs)Following Ionizing Radiation Treatment
The Ubiquitinated Form Localizes In The Nucleus In A Ndfip1-Dependent Manner
Alternative Antibody Names Anti-Brca1-Associated Atm Activator 1 antibody
Anti-Brca1-Associated Protein Required For Atm Activation Protein 1 antibody
Anti-BRAT1 antibody
Anti-BAAT1 antibody
Anti-C7orf27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance