• Western blot analysis of extracts of various cell lines, using BTNL2 antibody (STJ29200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BTNL2 antibody (246-455) (STJ29200)

SKU:
STJ29200

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BTNL2 (246-455) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTNL2
Gene ID: 56244
Uniprot ID: BTNL2_HUMAN
Immunogen Region: 246-455
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 246-455 of human BTNL2 (NP_062548.1).
Immunogen Sequence: LKVNGPSQPILVRVGEDIQL TCYLSPKANAQSMEVRWDRS HRYPAVHVYMDGDHVAGEQM AEYRGRTVLVSDAIDEGRLT LQILSARPSDDGQYRCLFEK DDVYQEASLDLKVVSLGSSP LITVEGQEDGEMQPMCSSDG WFPQPHVPWRDMEGKTIPSS SQALTQGSHGLFHVQTLLRV TNISAVDVTCSISIPFLGEE KIATFSLSGW
Tissue Specificity Expressed in brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis and thymus.
Function Negative regulator of T-cell proliferation.
Protein Name Butyrophilin-Like Protein 2
Btl-Ii
Database Links Reactome: R-HSA-8851680
Cellular Localisation Membrane
Single-Pass Type Ii Membrane Protein
Isoform 2 Is Present In The Nuclear
Vesicle And Plasma Membranes
Isoform 3 Is Found In Cytoplasmic Vesicle Structures And Is Not Membrane Bound
Alternative Antibody Names Anti-Butyrophilin-Like Protein 2 antibody
Anti-Btl-Ii antibody
Anti-BTNL2 antibody

Information sourced from Uniprot.org

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