• Immunofluorescence analysis of NIH/3T3 cells using C7 Rabbit polyclonal antibody (STJ27347) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using C7 Rabbit polyclonal antibody (STJ27347) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Mouse plasma, using C7 antibody (STJ27347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using C7 antibody (STJ27347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-C7 antibody (22-270) (STJ27347)

SKU:
STJ27347

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C7 (22-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C7
Gene ID: 730
Uniprot ID: CO7_HUMAN
Immunogen Region: 22-270
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 22-270 of human C7 (NP_000578.2).
Immunogen Sequence: ASSPVNCQWDFYAPWSECNG CTKTQTRRRSVAVYGQYGGQ PCVGNAFETQSCEPTRGCPT EEGCGERFRCFSGQCISKSL VCNGDSDCDEDSADEDRCED SERRPSCDIDKPPPNIELTG NGYNELTGQFRNRVINTKSF GGQCRKVFSGDGKDFYRLSG NVLSYTFQVKINNDFNYEFY NSTWSYVKHTSTEHTSSSRK RSFFRSSSSSSRSYTSHTNE IHKGKSYQLLVVENTVEVA
Post Translational Modifications C7 has 28 disulfide bridges. C-, N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
Protein Name Complement Component C7
Database Links Reactome: R-HSA-166665
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement Component C7 antibody
Anti-C7 antibody

Information sourced from Uniprot.org

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