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Anti-Cbl antibody

[STJ92057] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Cbl antibody
Short Description Rabbit polyclonal to Cbl.
Description Cbl is a protein encoded by the CBL gene which is approximately 99,6 kDa. Cbl is localised to the cytoplasm and cell membrane. It is involved in negative regulation of FGFR1 signalling, signalling by EGFR in cancer and the T cell receptor signalling pathway. It is an adapter protein that functions as a negative regulator of many signalling pathways that are triggered by activation of cell surface receptors. It accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Cbl is expressed in blood and cells of the nervous system. Mutations in the CBL gene may result in a Noonan syndrome-like disorder. STJ92057 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Cbl protein.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Specificity Cbl Polyclonal Antibody detects endogenous levels of Cbl protein.
Protein Name E3 ubiquitin-protein ligase CBL
Casitas B-lineage lymphoma proto-oncogene
Proto-oncogene c-Cbl
RING finger protein 55
RING-type E3 ubiquitin transferase CBL
Signal transduction protein CBL
Immunogen Synthesized peptide derived from human Cbl around the non-phosphorylation site of Y674.
Immunogen Region 610-690 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 867
Gene Symbol CBL
Molecular Weight 120 kDa
Database Links HGNC:1541
OMIM:165360
Alternative Names E3 ubiquitin-protein ligase CBL antibody
Casitas B-lineage lymphoma proto-oncogene antibody
Proto-oncogene c-Cbl antibody
RING finger protein 55 antibody
RING-type E3 ubiquitin transferase CBL antibody
Signal transduction protein CBL antibody2 antibody
RNF55 antibody
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Post-translational Modifications Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 . Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. Ubiquitinated, leading to its degradation via the proteasome.
Cellular Localization Cytoplasm. Cell membrane.. Colocalizes with FGFR2 in lipid rafts at the cell membrane.
Sequence and Domain Family The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.; The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
Swiss-Prot Key P22681_HUMAN
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