• Immunohistochemistry analysis of paraffin-embedded mouse testis using Cyclin G1 antibody (STJ27245) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using Cyclin G1 antibody (STJ27245) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cyclin G1 antibody (STJ27245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCNG1 antibody (200-295) (STJ27245)

SKU:
STJ27245

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCNG1 (200-295) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNG1
Gene ID: 900
Uniprot ID: CCNG1_HUMAN
Immunogen Region: 200-295
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-295 of human Cyclin G1 (NP_004051.1).
Immunogen Sequence: PSVLALSIIALEIQAQKCVE LTEGIECLQKHSKINGRDLT FWQELVSKCLTEYSSNKCSK PNVQKLKWIVSGRTARQLKH SYYRITHLPTIPEMVP
Tissue Specificity High levels in skeletal muscle, ovary, kidney and colon.
Function May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.
Protein Name Cyclin-G1
Cyclin-G
Database Links Reactome: R-HSA-6804757
Cellular Localisation Nucleus
Dna Replication Foci After Dna Damage
Alternative Antibody Names Anti-Cyclin-G1 antibody
Anti-Cyclin-G antibody
Anti-CCNG1 antibody
Anti-CCNG antibody
Anti-CYCG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance