• Immunohistochemistry analysis of paraffin-embedded rat brain using Cyclin K Rabbit polyclonal antibody (STJ112299) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Cyclin K Rabbit polyclonal antibody (STJ112299) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Cyclin K Rabbit polyclonal antibody (STJ112299) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cyclin K Antibody (STJ112299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCNK antibody (1-160) (STJ112299)

SKU:
STJ112299

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCNK (1-160) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNK
Gene ID: 8812
Uniprot ID: CCNK_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human Cyclin K (NP_001092872.1).
Immunogen Sequence: MKENKENSSPSVTSANLDHT KPCWYWDKKDLAHTPSQLEG LDPATEARYRREGARFIFDV GTRLGLHYDTLATGIIYFHR FYMFHSFKQFPRYVTGACCL FLAGKVEETPKKCKDIIKTA RSLLNDVQFGQFGDDPKEEV MVLERILLQTIKFDLQVEHP
Tissue Specificity Widely expressed. Highest levels in testis.
Function Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
Protein Name Cyclin-K
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-167152
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-2173796
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6807505
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclin-K antibody
Anti-CCNK antibody
Anti-CPR4 antibody

Information sourced from Uniprot.org

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