• Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Rat lung, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD27 rabbit polyclonal antibody (STJ22984) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Jurkat cells using CD27 antibody (STJ22984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat thymus using CD27 antibody (STJ22984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse thymus using CD27 antibody (STJ22984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using CD27 rabbit polyclonal antibody (STJ22984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Raji cells using CD27 rabbit polyclonal antibody (STJ22984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD27 antibody (20-191) (STJ22984)

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STJ22984

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD27 (20-191) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD27
Gene ID: 939
Uniprot ID: CD27_HUMAN
Immunogen Region: 20-191
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).
Immunogen Sequence: ATPAPKSCPERHYWAQGKLC CQMCEPGTFLVKDCDQHRKA AQCDPCIPGVSFSPDHHTRP HCESCRHCNSGLLVRNCTIT ANAECACRNGWQCRDKECTE CDPLPNPSLTARSSQALSPH PQPTHLPYVSEMLEARTAGH MQTLADFRQLPARTLSTHWP PQRSLCSSDFIR
Tissue Specificity Found in most T-lymphocytes.
Post Translational Modifications Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein Name Cd27 Antigen
Cd27l Receptor
T-Cell Activation Antigen Cd27
T14
Tumor Necrosis Factor Receptor Superfamily Member 7
Cd Antigen Cd27
Database Links Reactome: R-HSA-5669034
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd27 Antigen antibody
Anti-Cd27l Receptor antibody
Anti-T-Cell Activation Antigen Cd27 antibody
Anti-T14 antibody
Anti-Tumor Necrosis Factor Receptor Superfamily Member 7 antibody
Anti-Cd Antigen Cd27 antibody
Anti-CD27 antibody
Anti-TNFRSF7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance