• Western blot analysis of extracts of various cell lines, using CDK5RAP3 antibody (STJ115094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK5RAP3 antibody (1-300) (STJ115094)

SKU:
STJ115094

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDK5RAP3 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK5RAP3
Gene ID: 80279
Uniprot ID: CK5P3_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK5RAP3 (NP_788276.1).
Immunogen Sequence: MEDHQHVPIDIQTSKLLDWL VDRRHCSLKWQSLVLTIREK INAAIQDMPESEEIAQLLSG SYIHYFHCLRILDLLKGTEA STKNIFGRYSSQRMKDWQEI IALYEKDNTYLVELSSLLVR NVNYEIPSLKKQIAKCQQLQ QEYSRKEEECQAGAAEMREQ FYHSCKQYGITGENVRGELL ALVKDLPSQLAEIGAAAQQS LGEAIDVYQASVGFVCESPT EQVLPMLRFVQKRGNSTVY
Tissue Specificity Ubiquitously expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
Post Translational Modifications May be phosphorylated by CDK5. Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1. May be ufmylated. Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.
Function Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1. Required for liver development. (Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.
Protein Name Cdk5 Regulatory Subunit-Associated Protein 3
Cdk5 Activator-Binding Protein C53
Lxxll/Leucine-Zipper-Containing Arf-Binding Protein
Protein Hsf-27
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Colocalizes And Associates With Microtubules
Alternative Antibody Names Anti-Cdk5 Regulatory Subunit-Associated Protein 3 antibody
Anti-Cdk5 Activator-Binding Protein C53 antibody
Anti-Lxxll/Leucine-Zipper-Containing Arf-Binding Protein antibody
Anti-Protein Hsf-27 antibody
Anti-CDK5RAP3 antibody
Anti-IC53 antibody
Anti-LZAP antibody
Anti-MSTP016 antibody
Anti-OK antibody
Anti-SW-cl.114 antibody
Anti-PP1553 antibody

Information sourced from Uniprot.org

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