• Western blot analysis of extracts of various cell lines, using CEBPE antibody (STJ110350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CEBPE antibody (STJ110350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CEBPE antibody (1-190) (STJ110350)

SKU:
STJ110350

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CEBPE (1-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEBPE
Gene ID: 1053
Uniprot ID: CEBPE_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CEBPE (NP_001796.2).
Immunogen Sequence: MSHGTYYECEPRGGQQPLEF SGGRAGPGELGDMCEHEASI DLSAYIESGEEQLLSDLFAV KPAPEARGLKGPGTPAFPHY LPPDPRPFAYPPHTFGPDRK ALGPGIYSSPGSYDPRAVAV KEEPRGPEGSRAASRGSYNP LQYQVAHCGQTAMHLPPTLA APGQPLRVLKAPLATAAPPC SPLLKAPSPA
Tissue Specificity Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Post Translational Modifications Phosphorylated.
Function Transcriptional activator. C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation.
Protein Name Ccaat/Enhancer-Binding Protein Epsilon
C/Ebp Epsilon
Database Links Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ccaat/Enhancer-Binding Protein Epsilon antibody
Anti-C/Ebp Epsilon antibody
Anti-CEBPE antibody

Information sourced from Uniprot.org

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