• Western blot analysis of extracts of various cell lines, using CENPH antibody (STJ110670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CENPH antibody (1-247) (STJ110670)

SKU:
STJ110670

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CENPH (1-247) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPH
Gene ID: 64946
Uniprot ID: CENPH_HUMAN
Immunogen Region: 1-247
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CENPH (NP_075060.1).
Immunogen Sequence: MEEQPQMQDADEPADSGGEG RAGGPPQVAGAQAACSEDRM TLLLRLRAQTKQQLLEYKSM VDASEEKTPEQIMQEKQIEA KIEDLENEIEEVKVAFEIKK LALDRMRLSTALKKNLEKIS RQSSVLMDNMKHLLELNKLI MKSQQESWDLEEKLLDIRKK RLQLKQASESKLLEIQTEKN KQKIDLDSMENSERIKIIRQ NLQMEIKITTVIQHVFQNLI LGSKVNWAEDPALKEIVLQ
Function Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Protein Name Centromere Protein H
Cenp-H
Interphase Centromere Complex Protein 35
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-606279
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Associates With Active Centromere-Kinetochore Complexes Throughout The Cell Cycle
Colocalizes With Inner Kinetochore Plate Proteins Cenpa And Cenpc During Both Interphase And Metaphase
Alternative Antibody Names Anti-Centromere Protein H antibody
Anti-Cenp-H antibody
Anti-Interphase Centromere Complex Protein 35 antibody
Anti-CENPH antibody
Anti-ICEN35 antibody

Information sourced from Uniprot.org

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