• Western blot analysis of extracts of various cell lines, using CFL1 antibody (STJ26176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CFL1 antibody (1-100) (STJ26176)

SKU:
STJ26176

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CFL1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFL1
Gene ID: 1072
Uniprot ID: COF1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1).
Immunogen Sequence: MASGVAVSDGVIKVFNDMKV RKSSTPEEVKKRKKAVLFCL SEDKKNIILEEGKEILVGDV GQTVDDPYATFVKMLPDKDC RYALYDATYETKESKKEDLV
Tissue Specificity Widely distributed in various tissues.
Post Translational Modifications Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin.this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
Protein Name Cofilin-1
18 Kda Phosphoprotein
P18
Cofilin - Non-Muscle Isoform
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-399954
Reactome: R-HSA-5627117
Reactome: R-HSA-8950505
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Lamellipodium Membrane
Lamellipodium
Growth Cone
Axon
Colocalizes With The Actin Cytoskeleton In Membrane Ruffles And Lamellipodia
Detected At The Cleavage Furrow And Contractile Ring During Cytokinesis
Almost Completely In Nucleus In Cells Exposed To Heat Shock Or 10% Dimethyl Sulfoxide
Alternative Antibody Names Anti-Cofilin-1 antibody
Anti-18 Kda Phosphoprotein antibody
Anti-P18 antibody
Anti-Cofilin - Non-Muscle Isoform antibody
Anti-CFL1 antibody
Anti-CFL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance