• Western blot analysis of extracts of various cell lines, using CGREF1 antibody (STJ117044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CGREF1 antibody (20-200) (STJ117044)

SKU:
STJ117044

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CGREF1 (20-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CGREF1
Gene ID: 10669
Uniprot ID: CGRE1_HUMAN
Immunogen Region: 20-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human CGREF1 (NP_006560.3).
Immunogen Sequence: APKDGVTRPDSEVQHQLLPN PFQPGQEQLGLLQSYLKGLG RTEVQLEHLSREQVLLYLFA LHDYDQSGQLDGLELLSMLT AALAPGAANSPTTNPVILIV DKVLETQDLNGDGLMTPAEL INFPGVALRHVEPGEPLAPS PQEPQAVGRQSLLAKSPLRQ ETQEAPGPREEAKGQVEARR E
Post Translational Modifications Probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides.
Function Mediates cell-cell adhesion in a calcium-dependent manner. Able to inhibit growth in several cell lines.
Protein Name Cell Growth Regulator With Ef Hand Domain Protein 1
Cell Growth Regulatory Gene 11 Protein
Hydrophobestin
Cellular Localisation Secreted
Alternative Antibody Names Anti-Cell Growth Regulator With Ef Hand Domain Protein 1 antibody
Anti-Cell Growth Regulatory Gene 11 Protein antibody
Anti-Hydrophobestin antibody
Anti-CGREF1 antibody
Anti-CGR11 antibody

Information sourced from Uniprot.org

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