• Western blot analysis of extracts of various cell lines, using CHAD antibody (STJ117184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHAD antibody (1-359) (STJ117184)

SKU:
STJ117184

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHAD (1-359) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAD
Gene ID: 1101
Uniprot ID: CHAD_HUMAN
Immunogen Region: 1-359
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human CHAD (NP_001258.2).
Immunogen Sequence: MVRPMLLLSLGLLAGLLPAL AACPQNCHCHSDLQHVICDK VGLQKIPKVSEKTKLLNLQR NNFPVLAANSFRAMPNLVSL HLQHCQIREVAAGAFRGLKQ LIYLYLSHNDIRVLRAGAFD DLTELTYLYLDHNKVTELPR GLLSPLVNLFILQLNNNKIR ELRAGAFQGAKDLRWLYLSE NALSSLQPGALDDVENLAKF HVDRNQLSSYPSAALSKLRV VEELKLSHNPLKSIPDNAF
Tissue Specificity Present in chondrocytes at all ages.
Function Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha(2)beta(1). May play an important role in the regulation of chondrocyte growth and proliferation.
Protein Name Chondroadherin
Cartilage Leucine-Rich Protein
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Chondroadherin antibody
Anti-Cartilage Leucine-Rich Protein antibody
Anti-CHAD antibody
Anti-SLRR4A antibody

Information sourced from Uniprot.org

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